基质辅助激光解析飞行时间质谱快速测定水解胶原蛋白粉分子量

Rapid Determination of Molecular Weight of Hydrolyzed Collagen by MALDI-TOF-MS

应用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)快速测定了水解胶原蛋白粉的分子量。分别以α-氰基-4-羟基肉桂酸、2,5-二羟基苯甲酸和芥子酸为基质,在正离子模式下对水解胶原蛋白粉分子质量分布进行测定。结果显示:芥子酸为最适宜基质,分析条件为线性方式和反射方式相结合,可准确、快速确定水解胶原蛋白粉的分子量分布。该方法优于其它传统的分子量测定方法。

The molecular weight of hydrolyzed collagen was determinated rapidly by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.α-cyano-4-hydroxycinnamic acid,2,5-dihydroxybenzoic acid and sinapic acid were used,respectively,as matrices in positive ion mode.The experimental results showed that sinapic acid was the best matrix for hydrolyzed collagen and the analysis of condition were in both linear mode and reflector mode.MALDI-TOF-MS method was superior to other traditional method for the determination of molecular weight of hydrolyzed collagen.