氧化苦参碱对青霉素致痫大鼠学习记忆及海马p-JNK表达的影响

Effect of Oxymatrine on Learning and Memory of Epileptic Rats and the Expression of p-JNK in Hippocampus

目的观察氧化苦参碱(Oxymatrine,OXY)对青霉素(Penicillin,PEN)致痫大鼠学习记忆和海马神经元p-JNK表达的影响。方法成年SD大鼠24只随机分为生理盐水对照组(Normal saline,NS)、癫痫模型组(Epilepsy,EP)、OXY组和JNK抑制剂组(SP600125,SP),腹腔注射青霉素诱导大鼠癫痫发作,观察大鼠行为学改变,Morris水迷宫检测大鼠空间学习和记忆,免疫组化测定海马组织内p-JNK阳性细胞数。结果与EP组对比,OXY组大鼠逃避潜伏期和穿越平台次数明显减少(P<0.05);海马组织内p-JNK阳性细胞数明显降低(P<0.05)。结论 OXY可能通过抑制JNK信号通路的活化发挥对癫痫大鼠在空间学习和记忆方面的保护作用。

Objective To observe the effect of oxymatrine on the expression of p-JNK in hippocampus and the spatial learning and memory of penicillin - induced epileptic rats, as well as to investigate its mechanisms. Methods 24 SD rats were randomly divided into four groups,which were normal saline control group （NS） , epilepsy group （ EP）, Oxymatraine group （OXY） and JNK inhibitor group （ SP600125, SP）. Penicillin was intraperitoneally injected in rats to establish epileptic animal model. Behavioral changes were observed and im- munohistochemical method was used to measure the number of positive - cell of p - JNK in hippocampus. The spatial learning and memory was tested by Morris Water Maze （ MWM ）. Results Compared with in EP group, the escape latency, swimming distance, times of crossing platform in OXY group were significantly descreased （P 〈 0.05） ; the expression of p - JNK was less in OXY group than that in EP group （ P 〈 0.05 ）. Conclusion The protection of OXY for the spatial learning and memory in epileptic rats is performed by affecting the activation of JNK signaling pathway