益糖康对糖尿病大鼠主动脉NF-κBp65 mRNA含量、蛋白水平表达及NF-κB活化的影响

Effect of Yitangkang on content of aorta NF-κBp65 mRNA, protein expression and activation of NF-κB in diabetic rat

目的:探讨糖尿病大鼠在中药复方益糖康干预后主动脉NF-κBp65 mRNA含量、蛋白水平表达及NF-κB活化的变化。方法:将50只雄性Wistar大鼠随机分为正常对照组、模型组和中药复方益糖康治疗组。用高脂饲料和链脲佐菌素诱导糖尿病模型,中药煎剂益糖康对中药治疗组进行治疗性灌胃。治疗4周后取大鼠主动脉组织,检测主动脉NF-κBp65 mRNA含量、蛋白水平表达及NF-κB活化情况。结果:中药复方益糖康治疗组能明显降低NF-κBp65 mRNA含量,抑制蛋白水平表达及NF-κB活化,与模型组比较有统计学意义(P<0.01)。结论:益糖康可能通过对炎症核心转录因子NF-κB的影响来降低糖尿病大血管病变的风险。

Objective： Discussion on the change of content of aorta NF-κBp65 mRNA, protein expression and activation of NF-κB in diabetic rats after the intervention of TCM compound Yitangkang. Methods： The 50 male Wistar rats were randomly divided into 3 groups （normal group, model group, and TCM compound treatment group）. The model was made by intraperitoneally injected STZ, and TCM compound treatment group was administrated with Yitangkang orally for 4 weeks. After 4 weeks, the mRNA expression of nuclear factor NF-κBp65 in aorta was detected by PCR and Western. The activation of NF-κB in aorta was detected by immunise to chemistry staining. Results： TCM compound group inhibited the expression of NF-κBp65 and activation of NF-κB compared with model group in aorta （P0.05）. Conclusion： TCM Yitangkang can intervent the expression of NF-κBp65 and activation of NF-κB compared with model group in aorta on diabetic rats, than to reduce the risk of diabetes merge trunk pathological change.