祛浊通痹方对高尿酸模型大鼠血尿酸及黄嘌呤氧化酶的影响

Effect of Quzhuo Tongbi Recipe on levels of xanthine oxidase in hyperuricemia rats

目的:观察祛浊通痹方对高尿酸模型大鼠血尿酸及黄嘌呤氧化酶(xanthine oxidase,XOD)的影响,阐释祛浊通痹方治疗痛风改善尿酸代谢的作用机制。方法:通过喂养高钙饲料和腺嘌呤混悬液,制作大鼠痛风高尿酸血症模型,然后分为别嘌醇组(造模后灌胃别嘌醇)、中药组(造模后灌胃祛浊通痹方)、模型组(造模后不用药),另取正常大鼠为空白组(不造模不用药),分别于用药前及14d后取血检测各组血尿酸、黄嘌呤氧化酶水平。结果:痛风高尿酸血症模型大鼠血尿酸水平显著高于空白组(P<0.01);与治疗前比较,别嘌醇组、中药组血尿酸及XOD均明显下降(P<0.01)。治疗后各组间比较,模型组血尿酸及XOD高于别嘌醇组及中药组(P<0.01);别嘌醇组的XOD低于中药组(P<0.01)。结论:喂养高钙饲料和腺嘌呤混悬液能成功制作大鼠痛风高尿酸血症模型,祛浊通痹方能显著降低尿酸和抑制XOD的活性。

Objective： To observe effect of Quzhuo Tongbi Recipe on levels of xanthine oxidase in hyperuricemia rats for explaining the mechanism of Quzhuo Tongbi Recipe in improving the uric acid metabolism. Methods： By feeding high calcium diet and adenine suspension to set up rat model of hyperuricemia. Then they were randomly divided into allopurinol group （after modeling, administered allopurinol）, Chinese medicine group （after modeling, administered Quzhuo Tongbi Recipe）, model group （no treatment after modeling） and normal rats being the control group （no medication and no modeling）. Before and 14 days after treatment, the levels of uric acid and xanthine oxidase （XOD） were detected. Results： The levels of blood uric acid in hyperuricemia rats were significantly higher than the control group （P0.01） and 14 days after treatment, the levels of uric acid and XOD of allopurinol and Chinese medicine groups reduced significantly and lower than model group. The level of XOD of allopurinol group lower than Chinese medicine group （P0.01）. Conclusion： Suspension feeding high calcium diet and adenine can successfully set up hyperuricemia rat model and Quzhuo Tongbi Recipe can significantly reduce the uric acid and inhibit the activity of XOD.