清化瘀毒方对酒精性大鼠肝纤维化血清HA、LN、PCⅢ含量及TIMP-1的影响

Effect of Thanh Hoa (Qinghua Yudu Fang) on blood plasma HA、LN、PCШ levels and the expression of tissue inhibitor of metal protease-1 (TIMP-1) of hepatic fibrosis rat

目的:探讨清化瘀毒方对酒精性大鼠血清肝纤维化指标及组织金属蛋白酶抑制剂-1(TIMP-1)的作用机制。方法:采用以酒精为主导的复合因素建立酒精性大鼠肝纤维化模型,实验初随机分成正常对照组10只,造模组55只;造模成功后,造模组采用随机分成3组,分别为复方鳖甲软肝片组10只,清化瘀毒方组10只和模型空白组10只;于第20周末次给药后禁食,麻醉后打开腹腔,抽取腹主动脉血并离心检测AST、ALT、HA、LN、PCⅢ及TIMP-1的含量。结果:清化瘀毒方组AST、ALT、HA、LN、PCⅢ含量比模型空白组下降,有统计学意义(P<0.05);血清TIMP-1的表达清化瘀毒方组与模型空白组相比,有统计学意义(P<0.05)。结论:清化瘀毒方能改善肝功能,使血清中的肝纤维化指标下调,降低TIMP-1的蛋白表达升高,具有抗炎保肝、抗肝纤维化作用。

Objective： To discuss the mechanism of anti-alcoholic liver fibrosis function of Thanh Hoa （Qinghua Yudu Fang） and its validity and reliability in treating alcoholic liver fibrosis. To observe the effect of Thanb Hoa on the expression of hepatic fibrosis transforming growth factor-β1 （TGF-β1） and tissue inhibitor of metal protease-1（TIMP-1）. Methods： 65 SPF- level male Wistar rats were involved in the study, and were randomly divided into two groups, as 10 in the blank control group and 55 in the model group. After the success of modeling, the model group was randomly divided into 3 groups, as 10 in the compound Biejia Ruangan Tablet group, 10 in the Thanh Hoa group and 10 in the model control group. At the end of the 20th week, all the rats were sacrificed, test the ALT, AST, TIMP-1 with the plasma. Results： Compared with the model control group, the Thanh Hoa group had significant nigher scores of liver inflammation activity and liver fibrosis degree and AST, ALT and HA, LN, PClll （P〈0.05）. Compared with the model control group, the Thanh Hoa group had significant nigher expression of hepatic TIMP-1 （P〈0.05）. Conclusion： Thanh Hoa had the function of reducing liver inflammation activity, fibrosis degree and TIMP- 1,AST,ALT,HA,LN,PC111 expression of alcohol induced rat hepatic fibrosis, which maybe a mechanism of its anti-inflammatory and anti-alcoholic liver fibrosis features.