青蒿琥酯对MDA—MB-231细胞增殖抑制作用及其机制

Anti-proliferative effect of artesunate on MDA-MB-231 cell and its mechanisms

目的探讨青蒿琥酯（artesunate，ART）对乳腺癌雌激素受体阴性细胞MDA—MB-231抑制作用及其机制。方法ART处理细胞3d后，采用MTT比色法检测细胞增殖功能，观察细胞形态、结构的改变，免疫细胞化学检测Bax，nm23，Bcl-2，P21WAF1／CIP1蛋白的表达情况。结果ART作用于乳腺癌细胞MDA—MB-231后，发现其对细胞的抑制作用呈明显浓度依赖性，可导致细胞形态、结构改变；免疫细胞化学结果显示2μmol／L青蒿琥酯作用细胞72h后，药物组同细胞对照组比较，可上调Bax、nm23、P21WAF1／CIP1蛋白表达，差异有统计学意义（P〈0．05），Bcl-2蛋白质表达差异无统计学意义（P〉0．05）。结论ART有抑制MDA—MB-231细胞增殖的作用，其机制可能与上调Bax、nm23、P21WAF1／CIP1蛋白表达有关。

Objective To study the effects of artesunate （ART） on estrogen receptor negative breast cancer cell line MDA-MB-231 and its mechanisms. Methods Treated with ART for 3 days, MDA-MB-231 cells proliferation was examined by MTT assay. The morphological and uhrastructural changes of MDA-MB-231 cells were observed under microscope and electronic microscope. Immunocytoehemistry was used to detect the expression of Bax, nm23, Bcl-2, and P21WAF1/CIP1. Results ART treatment led to a dose dependent inhibition of MDA-MB-231 cells. ART could change the morphology and ultrastmcture of MDA-MB-231 cells. After treatment with ART （2μmol/L） for 72 hours, immunocytochemical staining showed that the expression of Bax, nm23, and P21WAF1/CIP1 was upregulated in comparison to the control group （P 〈 0. 05） while the expression of Bcl-2 in MDA-MB-231 cells didn＇t have a significant change （ P 〉 0. 05 ）. Conclusions ART shows an anti-proliferative effect on MDA-MB-231 cells. The mechanisms may be related to upregulation of Bax, nm23 and P21WAF1/CIPI expression.