香砂和中丸中橙皮苷的HPLC测定

Determination of Hesperidin in Xiangsha Hezhong Wan by HPLC

目的建立香砂和中丸中橙皮苷的含量测定方法。方法采用高效液相色谱法,Diamonsil ODS1 C18色谱柱,以甲醇-醋酸-水(35∶4∶61)为流动相,流速为1.0 mL/min,检测波长为283 nm。结果橙皮苷在0.42～8.4μg范围内呈良好线性,回归方程为Y=830 125.1X+5 849.875,r=0.999 8,平均加样回收率为99.35%,RSD=0.68%(n=6)。结论本方法简便、准确、专属性强,测定结果重复性好。

Objective To establish the method for detemining the content of Hesperidin in Xiangsha Hezhong Wan by HPLC.Methods Diamonsil ODS1 C18 column was used with methanol-acetic acid-water（35∶4∶61）as the mobile phase,detection wavelength as 283 nm,and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.42-8.4μg for Hesperidin and linear equation was Y= 830 125.1X＋5 849.875,r=0.999 8.The average recovery was 99.35%and the related standard deviation（RSD）was 0.68%（n=6）.Conclusion This method was simple,accurate and proper,and the reduplication of the result was good.It provides quantitative analysis method for Hesperidin in Xiangsha Hezhong Wan.