牛蛙腐皮病病变皮肤亚显微结构的观察

Observation on Ultrastructural Changes of the Affected Skin of Bull Frog Suffering from Putrid Skin Disease

为揭示牛蛙腐皮病的病理形态变化和发病的机理,采用透射电镜对病蛙不同病期的病变皮肤进行了观察.皮肤病变早期可见角化层脱落,鳞状上皮细胞连接疏松,桥粒减少;随着病情发展,细胞间隙增宽,桥粒进一步减少,细胞核固缩、溶解,细胞裂解;病变后期,表皮层崩解,真皮层胶原纤维溶解,炎症区有大量炎症细胞浸润,并可见吞噬了细菌的单核细胞与中性粒细胞.

The affected skin samples at different stages of putrid skin disease in bullfrog were observed by using TEM for discovering pathomorphological changes of bull frogs rotten skin disease and its mechanism.At the early stage of the disease,it was found that stratum corneum fell down,the cells conjuction of stratified epithelium became loose,desmosome lessened with the progress of disease;space between cells widened,desmosome further decreased and karyopyknosis,karyolysis and decomposition of the cells were found.At the later stage of the disease,collapse of the epidermis,lysis of collagenous,fiber of the dermis and infiltration of inflamatory cells with bacteriophagous monocytes and neutrophils were found in the inflamatory area of the skin of bull frog.