摘要
笔者采用分光光度法测定了日照绿茶中的总黄酮含量,并优化了分析方法.芦丁对照品浓度在8.5-42.4μg/ml之间,吸光值与浓度的线性关系为y=0.023 6x-0.0499,相关系数R=0.9994,加标平均回收率为101.85%,RSD=2.88%(n=3).用70%的乙醇水溶液,超声提取50min时总黄酮的提取率最高.测得日照绿茶的总黄酮含量为29.49m/g.
The total flavonoids in Rizhao Green Tea has been determined by spectrophotometery. The linear relationship of rutin was in the range of 8.5 - 42.4 μg/ml. The regression equation was y = 0. 023 6x - 0. 049 9, R =0. 999 4. The average recovery was 101.85%, RSD = 2.88% ( n = 3 ). The results showed that the content of flavonoids was measured 29.49 mg/g, among the highists in several kinds of common tea from market.
出处
《山东师范大学学报(自然科学版)》
CAS
2011年第2期73-75,共3页
Journal of Shandong Normal University(Natural Science)
基金
山东省教育厅项目(2009310).
关键词
日照绿茶
总黄酮
分光光度法
Rizhao green tea
flavonoids
spectrophotometry