摘要
目的 调查西安地区职业献血员及血透患者和不同类型肝炎患者TTV(transfusion transmitted virus) 感染状况.方法 在TTV ORF1 区设计两套引物,用巢式聚合酶链反应(nestedPCR) ,检测我院有偿献血员208 例,血液透析患者30例及我院住院的非肝炎患者128 例,不同类型肝炎患者147 例TTV 感染率.结果 西安地区职业献血员TTV 阳性率16-8 % (35/ 208) ,血透患者TTV 阳性率47 % (14/ 30) ,非肝炎患者TTV 阳性率2-2 % (3/ 128 ) , 前两组与后一组统计处理(χ2 = 14-26 )(χ2 = 47-28) (χ2 = 49-48 ,P< 0-001) ,整体比较(χ2 = 41-886 ,P< 0-001) . 非甲- 戊型肝炎60 % (9/ 15) , 庚型肝炎组为50 % (6/ 12) ,乙型肝炎组为26 % (21/ 80) ,丙型肝炎组为19 %(6/ 31) ,甲型肝炎组0(0/ 9) .结论 西安地区与血液紧密接触人群中存在严重的TTV 感染,其分布支持TTV 以输血传播为主特性. 不同类型肝炎患者中,除甲肝外均有TTV 重叠感染,其中以非甲- 非戊型肝炎和庚型肝?
AIM Detection of transfusion transmitted virus (TTV) infection and clinical investigation in blood donors, patients with defferent kinds of hepatitis and hemodialysis patients in Xi'an . METHODS A nested polymerase chain reaction (nested PCR) assay with two pair of primers from OFR1 of TTV genome was established to detect TTV DNA in the serum samples from 208 cases of blood donors, 128 cases of patients without hepatitis, 147 cases of patients with defferent kinds of hepatitis and 30 cases of hemodialysis patients in Xi'an. RESULTS TTV DNA was detected in serum from 35 of 208(16 8%) blood donors, 14 of 30 (47%) hemodialysis patients, 9 of 15 (60%) non A non E hepatitis, 6 of 12 (50%) hepatitis G, 21 of 80 (26%) hepatitis B, 6 of 31 (19%) hepatitis C, 0 of 9 (0%) hepatitis A, 3 of 128 (2 3%) non hepatitis patients in Xi'an. CONCLUSION The result of the study suggests that TTV infection is common in blood donors and hemodialysis patients in Xi'an. Blood donors and hemodialysis patients are the population at high risk for TTV infection. Non A non E hepatitis and hepatitis G is the population at most high risk for TTV infection. TTV may have pathogenicity.
出处
《世界华人消化杂志》
CAS
1999年第11期967-969,共3页
World Chinese Journal of Digestology