正交拉丁方实验在猕猴桃组织培养中的应用

THE APPLICATION OF ORTHOGONAL LATIN SQUARE EXPERIMENT IN THE CULTURE IN VITRO OF CHINESE GOOSEBERRY

本文应用正交试验法对中华猕猴桃组织培养过程中培养基的Z_t、K_t和NAA浓度进行了优选。试验结果表明:Z_t的浓度变化对愈伤组织诱导率、平均丛生芽数以及平均愈伤组织重量均有极显著的影响,而K_t和NAA的浓度变化不显著。对愈伤组织诱导率的最佳配方为1.5mg/L Z_t+0.05mg/L K_t+0.95mg/L(?)NAA;对平均丛生芽数和平均愈伤组织重量的最佳配方为2.9mg/L Z_t+0.05mg/L K_t+1.5mg/L NAA。

The optimum selection of plant regulator concentrations was carried out by means of orthogonal experiment in Chinese gooseberry (Actinidia chinensis)culture in vitro. The results have shown that the changes of Zt concentration have significant effects on the percentage of callus induced, the avarage weight of callus and the avarage number of multiple-shoots, but there was no singnificance in the changes of concentrations of Kt and NAA. To percentage of callus induction, the best combinations of plant regulators were 1.5mg/L Zt+0.05mg/L Kt+0.95mg/L NAA; to avarage number of multiple-shoots and avarage weight of callus, the best combinations of plant regulators were 2.9mg/L Zt+0.05mg/L Kt+1.5mg/L NAA.