束缚应激对大鼠脑组织Na^+,K^+-ATP酶活性的影响

The Effect of Immobilization Stress on Activity of Na +,K +-ATPase in Rat Brain Tissue

目的：探讨心理应激源对脑组织Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性的影响及发生机制，以及自由基清除剂对该酶活性的保护作用。方法：将大鼠分为对照组、束缚组和治疗组。用酶组化法制片，用图像分析仪测脑组织Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶的活性；用ＴＡＢ法测ＭＤＡ的含量。结果：束缚组和治疗组的Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性明显低于对照组，ＭＤＡ含量明显高于对照组；治疗组Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性明显高于束缚组，ＭＤＡ含量明显低于束缚组；束缚组、治疗组的ＭＤＡ含量与Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性呈直线负相关。结论：较长时间的束缚应激可致大鼠脑组织Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性降低；该酶活性降低与氧自由基损伤有关；给予ＳＯＤ＋ ＣＡＴ治疗。

To study the mechanisms of effect on activity of Na +,K +-ATPase in brain tissue by psychic stressors and the protecting function of free radical enzymatic scavengers. Methods:Experimental rats were divided into 3 groups: control group, immobilization group and group of administration of SOD and CAT (administration group). Section preparations were prepared by enzymo-histochemistry. Activity of Na +,K +-ATPase in Brain tissue was indicated by grey scale value detected by an image-analysing instrument. MDA content in brain tissue was measured by TBA method and protein in the tissue was quantitatively analysed by biuret method.Results: Activity of Na +,K +-ATPase is lower while MDA content is higher in immobilization and administration groups than in control group. Activity of Na +,K +-ATPase is obviously higher while MDA content is lower in administration group than in immobilization group. MDA content and activity of Na +,K +-ATPase are rectilinear and negatively correlative. Conclusion: Long-term immobilization stress caused decrease in activity of Na +,K +-ATPase in rat brain tissue. The decrease is relevant to lesion of oxygen free radicals. SOD and CAT had protective function in preserving activity of Na +,K +-ATPase.