不明原因男性不育患者精子核DNA与形态定量研究

Study of sperm core DNAquantity and form in m ale sterility

目的：探讨不明原因男性不育患者精子核ＤＮＡ定量及精子形态的变化情况。方法：对５０ 例正常生育男性和３５ 例不明原因不育患者精液进行精子核ＤＮＡ积分吸光度、平均灰度和精子面积、核长、核宽、周长、圆度、尾长等定量分析。结果：不育患者精子核ＤＮＡ 积分吸光度明显减低（Ｐ＜ ０．０１），平均灰度明显增高（ Ｐ＜ ０．０１），面积、核长、核宽、周长、圆度、尾长与生育男性比较，无显著性差异（ Ｐ＞ ０．０５）。结论：不明原因男性不育患者精子核ＤＮＡ积分吸光度明显减低。

Purpose:In order to study the unknoun reason m ale sterility patient′s sperm core DNA quantity and the circum stanlce change aboutthe sperm form .Methods:The sem en of50 nor- m al fertility′s m ale w as com pared w ith 35 unknow n reason of sterility patient′s,analysing sperm core DNA integralopticaldensity,average grey m atter,the quantity and form aboutsperm ′s area, core′s length,core′s w idth perim eter circum ference tail′s length,etc.Results:Sterility patient′s sperm core DNAintegralopticaldensity had a clearreduction ( P< 0.01);averagegrey m atter had a clear increase ( P < 0.01),area core′s length core′s w idth perim eter circum ference and tail′s length contrast w ith fertilitys m ale′s had no significant difference (P > 0.05).Conclusions:The unknoun reason m ale sterility patient′s sperm core DNA integralopticaldensity had a clear reduc- tion.