拟南芥电压依赖型阴离子通道参与水杨酸信号应答

Voltage-dependent Anion Channels of Arabidopsis thaliana Involved in Salicylic Acid Signaling Pathway

电压依赖型阴离子通道蛋白(voltage-dependent anion channels,VDAC)是线粒体外膜分布的重要蛋白,参与形成线粒体通透性转换孔,控制线粒体内外的物质进出。以RLD拟南芥为材料,构建VDAC过量表达和抑制表达的转基因株系,初步探索VDAC在水杨酸(Salicylic acid,SA)信号传递途径中的作用。对转基因拟南芥种子进行SA处理的萌发实验,发现VDAC影响拟南芥种子对SA的敏感性:过量表达株系种子对SA敏感,萌发率较低,而抑制表达株系种子敏感性较低,萌发率高。用实时荧光定量PCR检测SA信号传递的Marker基因PR1,发现在过量表达株系中,伴随VDAC水平升高,PR1上调;同样在抑制表达株系中,VDAC降低,PR1下降。由此说明,VDAC参与了拟南芥SA信号传递。

Voltage-dependent anion channels（VDAC） proteins distribute in mitochondrial outer membrane.They participate in the formation of mitochondrial permeability transition pore（PTP） and control substances in and out of the mitochondria.To explore the possibility of VDAC in SA signaling pathway,Arabidopsis thaliana（RLD） was taken as experiment material,and transgenic plant lines of VDAC over-expression and inhibited expression were gotten.Then germination experiment of transgenic plant seeds were treated with SA.The result showed that VDAC affected the sensitivity to SA of transgenic seeds.The over-expression lines seeds were sensitive to SA,with low germination rate.In contrast,the inhibited expression lines were less sensitive and the germination rate was high.PR1,marker gene of SA signaling pathway,was used for the real-time fluorescence quantitative PCR.It was found that in over-expression lines,accompanied by VDAC high level,PR1 increased;and in the inhibition lines,VDAC lower level,PR1 declined.In summary,Arabidopsis thaliana VDAC was involved in SA signaling pathway.