色氨酸羟化酶2基因和家庭环境因素与反社会人格障碍的关联分析

Association of tryptophan hydroxylase-2 gene and family environment with antisocial personality disorder

目的 探讨色氨酸羟化酶2（TPH2）基因、家庭环境因素及其交互作用与反社会人格障碍（ASPD）的关系。方法选取TPH2基因rs4290270和rs73051152个多态性位点，采用聚合酶链反应一限制性片段长度多态性基因分型技术，测定117例反社会人格障碍患者（ASPD组）和142名健康人（对照组）的TPH2基因多态性分布，并运用家庭环境量表一中文版（FamilyEnvironmentScale—ChineseVersion，FES—CV）评估家庭环境。结果ASPD组TPH2基因rs4290270、rs73051152个多态性位点的基因型和等位基因频率分布与对照组比较，差异均无统计学意义（P〉0．05）。ASPD组TA单体型频率显著高于对照组，差异有统计学意义（X^2=6．177，P〈0．05），相对危险度的估计值（OR）为1．865，95％可信区间（凹）为1．135～3．065；其他单体型在2组间的差异无统计学意义。家庭环境中的情感表达和道德宗教观2个因子与TA单体型存在交互作用（P〈0．05），OR值分别为1．122和1．080，95％C／分别为1．043—1．206和1．010～1．155。结论TPH2单体型TA可能与ASPD的发生有关，负性的家庭环境可能进一步加重携带危险单体型对个体的不利影响，个体发生反社会人格障碍的易感性更高。

Objective To study the association of tryptophan hydroxylase 2 （TPH2） gene polymorphism and family environment with antisocial personality disorder （ASPD） in Chinese Han population. Methods The single nuelentide polymorphism （SNPs） of TPH2, rs4290270 and rs7305115 were analyzed by PCR-RFLP genotyping assay in 117 ASPD patients and 142 healthy controls. The family Environment Scale-Chinese Version （FES-CV） was used to evaluate the family environment of all subjects. Results There were no significant differences between ASPD and controls in genotype and allele frequencies of rs4290270 and rs7305115. The distributions of TA haplotype was significantly more frequent in patients than in controls [ odds ratio （OR） 1. 865, 95% confidence interval （CI） 1. 135 - 3. 065 ,P 〈 0. 05 ]. Interactions between genetic and environmental （ G ×E） revealed that expressiveness and moralreligious emphasis increased the risk of ASPD in the presence of TA haplotype [ OR = 1. 122, 95% CI 1. 043 - 1. 206; OR = 1. 080, 95% CI 1.010 - 1. 155, P 〈 0. 05 ] . Conclusion The results suggest that TA haplotype might be with higher susceptibility for ASPD and adversity family environments might increase the risk of ASPD in ones with risk haplotype.