摘要
建立龙胆提取物的高效液相色谱指纹图谱。以龙胆提取物为研究对象,应用反相高效液相色谱法,色谱柱为Hypersil ODS(4.6mm×250mm,5μm),甲醇-水二元梯度洗脱模式,流速为1.0m/min,检测波长为270nm,建立了10批不同批次的龙胆提取物的HPLC指纹图谱。应用"中药色谱指纹图谱相似度评价系统"(2004A版)对各批次龙胆提取物进行了质量评价。该方法简便、可靠,可用于龙胆提取物的真伪鉴别和质量评价。
To establish the RP-HPLC fingerprint analysis for the quality control of Gentianae Radix et Rhizoma.HPLC fingerprint analy-sis method for Gentianae Radix et Rhizoma was developed,Hypersil ODS5μm（4.6 mm×250 mm） was used,with mixture of methanol and water as mobile phase in a gradient mode.The flow rate was 1.0 mL/min.The wavelength of measurement was 270 nm.The samples’ quality was assessed by Similarity Evaluation System for Chromato-graphic Finger print of TCM2004.The method can be used to identify and evaluate the quality Gentianae Radix et Rhizoma.
出处
《特产研究》
2011年第2期22-24,46,共4页
Special Wild Economic Animal and Plant Research
基金
国家科技部支撑计划项目(2007BAI38B03)
