用光子标记技术实时动态观测中性粒细胞吞噬病原体细胞

IMAGING THE PROCESS OF NEUTROPHILIC GRANULOCYTE PHAGOCYTOSIS OF TRANSFORMEDBL_(21) WITH PHOTONIC MARKING TECHNIQUE 

基因标记和光子学显象技术相结合，实时观测中性粒细胞吞噬病原体细胞骨架运动规律． 利用致冷ＣＣＤ系统组建了高灵敏度的荧光激发与测试装置． 将带有绿色荧光蛋白（ＧＦＰ）基因的ＰＲＳＥＴＢ质粒转化大肠杆菌ＢＬ２１，利用ＢＬ２１高效表达ＧＦＰ后发出强烈绿色荧光的特点，用光子显象技术连续记录荧光的变化，从而在不受干扰的条件下实时动态观察了中性粒细胞粘附、内吞、消化大肠杆菌的全过程．

Photonic imaging technique was combined with gene marking technique to record in vivo the process of neutrophilic granulocyte phagocytosis of transformed BL 21 A high sensitive fluorescence exciting and detecting system was constructed on the basis of a cooled CCD system Escherichia coli BL 21 was transformed with plasmid P RSETB that carried green fluorescent protein (GFP) gene The transformed BL 21 efficiently expressed GFP and emitted strong green fluorescence while exciting with blue light The whole phagocytosis process including:adhesion,ingestion,and exocytosis was thus tracked by measuring the fluorescence with the CCD camera