多发性乳腺纤维腺瘤患者免疫组化相关指标的研究

The Study on the Relative Immunohistochemical Index in the Patient with Multiple Fibroadenoma of Breast

目的:探讨多发性乳腺纤维腺瘤的病因和发病机理,研究免疫组化法检测的相关指标在本病发生过程中的意义。方法:用免疫组化法检测90例多发性乳腺纤维腺瘤患者和30例乳腺增生病患者乳腺组织中雌激素受体(estro-gen receptor,ER)、孕激素受体(progesterone receptor,PR)、细胞增殖抗原标记物(Ki-67)、抑癌基因P53及人类癌基因CerbB-2的表达。结果:(1)多发性乳腺纤维腺瘤患者PR的阳性表达率及高强度阳性表达率均高于对照组(前者P<0.01,后者P<0.05);Ki-67的阳性表达率明显高于对照组(P<0.05);(2)多发性乳腺纤维腺瘤患者ER、PR之间有显著相关性(P<0.01);(3)多发性乳腺纤维腺瘤患者ER的阳性表达率与对照组相比无显著性差异(P>0.05);(4)多发性乳腺纤维腺瘤患者ER、PR与Ki-67的表达之间无相关性(P>0.05);(5)P53和CerbB-2在本病中均无表达。结论:(1)局部乳腺组织中PR的阳性表达率及阳性表达强度升高也是乳腺纤维腺瘤发病的主要因素;(2)ER、PR间存在的相关性在本病的发生中起了主要作用;(3)Ki-67的阳性表达率升高,说明细胞增殖能力较强,这可能也是本病发生的直接原因。

Objective：To examine the pathogenesis of multiple fibroadenoma of breast and discuss the significance of immunohistochemical indicatrix in the process of the disease.Methods：The expression of estrogen receptor（ER）,progesterone receptor（PR）,the cell proliferating antigen Ki-67,anti-oncogene P53 and human oncogene CerbB-2 were tested by the immunohistochemical assay in 90 cases with multiple fibroadenoma of breast and 30 cases with mammary hyperplasia.Results：（1）The positive rate and the powerful positive rate of PR expression were both higher than that in healthy control group significantly（respectively P0.01,P0.05）.（2）There was significant dependability between ER and PR in the cases with multiple fibroadenoma of breast（P0.01）.（3）There was no significant dependability for the positive rate of ER expression between the cases with multiple fibroadenoma of breast and the control group（P0.05）.（4）There was no significant dependablity between the expression of ER and PR and the expression of Ki-67（P 0.05）.（5）There was no expression of P53 and CerbB-2 in the cases with multiple fibroadenoma of breast.Conclusion：（1）The increase of the positive rate and the powerful positive rate of PR expression in mammary gland partly is the major factor for the multiple fibroadenoma of breast.（2）The dependability between ER and PR make an important role in the disease.（3）The increase of the positive rate of Ki-67 expression prove that the cells＇ reproductive activity is strong,and perhaps this is the immediate cause which induce the disease.