摘要
目的:探讨间接比例法检测结核杆菌药物敏感性试验的影响因素。方法:通过对我室2009、2010年两次参加国家结核病参比室组织的结核杆菌药物敏感试验室间质评的结果进行比较与分析,找出影响实验成败的关键因素包括含药培养基配制的质量控制、实验过程的规范化、环境温度的实时监测、结果观察及判断等等,为以后的实验提供技术支持。结果:2010年与国家结核病参比室的药敏结果符合率比2009年有较大的提高。结论:培养基的质量、培养的温度和操作人员的技术娴熟程度均会影响药敏试验结果。
Objective：To explore the influence of indirect proportion method in testing Mycobacterium tuberculosis.Methods：Mycobacterium tuberculosis drug susceptibility testing results were compared and analyzed to identify the key factors of success or failure of experiments,including preparation of the quality of the drug-containing medium control,experimental process of standardization,real-time monitoring of the ambient temperature,the results of observations and judgments,etc.Results：2010 national TB reference room with susceptibility results consistent with a greater rate than the increase in 2009.Conclusion：The quality of the medium,culture temperature and skilled operators will affect the degree of susceptibility test results.
出处
《医学理论与实践》
2011年第10期1121-1123,共3页
The Journal of Medical Theory and Practice
基金
国家第五轮全球基金耐多药结核病防治项目
关键词
间接比例法
结核杆菌
药物敏感试验
影响因素
Indirect proportion method
Mycobacterium tuberculosis
Drug sensitivity test
Factors
