抗丝黑穗病玉米种质资源的SSR标记遗传多样性分析

Genetic Diversity Analysis of Maize Germplasm Resources against Sporosporium Reilianum with SSR Markers

采用SSR标记方法研究了40份对丝黑穗病有不同抗性玉米自交系的遗传多样性。选用57对SSR扩增稳定的引物,将自交系划分为唐四平头,旅大红骨,Lancaster,Reid,PA,PB这6个类群,结果与系谱来源一致性很高。其中,33个抗病或中抗材料分布于6个类群中,根据杂种优势利用原理,均可用于改良类群内的感病自交系和选育抗病自交系。尤其是旅大红骨群、Lancaster群和PB群中抗病自交系较多,可以构建抗病种质群体。

Simple sequence repeats（SSR） markers were adopted in heterotic parts of 40 maize inbred lines of different resistance to head smut.By 57 SSR primers giving stable amplified profiles,40 inbred lines are classified into 6 clusters,which are completely consistent with the heterotic groups determined by their pedigree information（I.e.Sipingtou,Luda Red Cob,PA,PB,Ried and Lancaster）.Cluster analysis showed that 33 head smut-resistance corn inbred lines could be classified into six distinct groups.According to the principle of heterosis utilization,resistant lines could be used to improve susceptible lines in the same group and to select new lines resistant to sporisorium reilianum.Especially in Luda Red Cob,Lancaster and PB,resistant lines were more,so resistant germplasm populations could be obtained.