大豆根瘤促生剂对费氏中华根瘤菌产结瘤因子影响

Effect of Producing Nod Factor from Sinorhizobium fredii Made by Soybean Nodule Promoting Preparation

以费氏中华根瘤菌(Sinorhizobium fredii)为研究对象,利用紫外分光光度计对大豆根瘤促生剂的作用进行了初步研究。结果表明:大豆根瘤促生剂可以使费氏中华根瘤菌的对数期提前,稳定期延长;同时,该根瘤促生剂可以诱导费氏中华根瘤菌产生结瘤因子;在根瘤促生剂存在的条件下,使用实验室培养的黄豆芽配制的培养基可以促进结瘤因子的产生;最佳的结瘤因子诱导剂为全豆芽研磨液+大豆根瘤促生剂,诱导剂的最佳用量为1.0%,最佳诱导温度为30℃。

In the study on Sinorhizobium fredii,spectrophotometer was applied to prove the effect of Soybean Nodule Promoting Preparation.The results were： This Soybean Nodule Promoting Preparation could advance and extend the log stage of Sinorhozobium fredii;meanwhile to make the Sinorhizobium fredii produce nod factor;Medium made by bean sprouts produced by ourselves could produce more nod factor;the best induced preparation was entire bean sprouts ground liquid and in this Soybean Nodule Promoting Preparation;the best amount of induce preparation was 1.0%;and the best temperature to produce nod factor was 30 ℃.