呈味核苷酸二钠结晶工艺研究

Study on Crystallization Process of Disodium 5'- Ribonucleotide

呈味核苷酸二钠包括5'-肌苷酸二钠和5'-鸟苷酸二钠,难以形成良好的结晶体,针对现有研究中存在的问题,对呈味核苷酸二钠结晶工艺进行改进,优化工艺参数。研究发现,采用乙醇为溶析剂,在合适的结晶液浓度和溶析剂添加量的条件下,添加晶种程序降温结晶,有利于提高结晶收率、产品纯度和堆密度。在优化的结晶条件下,呈味核苷酸二钠产品色谱纯度达到99.54%,堆密度达到0.54g/cm3,满足国际市场的要求。

Disodium 5＇-Ribonucleotide composed of disodium 5＇- inosinate and disodium 5＇- guanylate. The improvement on the crystalline technique of disodium 5＇- ribonucleotide was reported. Crystallization solvent was ethanol. Under a suitable condition of crystallization, the seed crystal was helpful to improve the crystallization yield, product purity and bulk density. Under the optimized conditions, the product could be obtained with purity of 99.54%, the bulk density was 0.54 g/cm3. The quality achieved the international market requirements.