基于SSR标记的杂交玉米金单999种子的纯度检验

Purity Identification of Jindan 999 Hybrid Maize Seed by SSR Marker

为快速、准确地判断杂交玉米金单999种子的纯度,有效阻止伪劣种子在市场上的流通,提高种子市场的种子质量,同时为其他杂交玉米种子的纯度鉴定提供方法参考,利用SSR标记技术,从30对SSR引物中筛选出能够清楚、稳定、准确地鉴别出金单999的亲本及杂交种且呈现双亲互补带型的2个引物,对金单999玉米杂交种进行纯度检验。结果表明:Bnlg1496、umc1222这2对引物完全可以对杂交玉米金单999的种子进行纯度检验,并检验出金单999种子的纯度为98.12%。

Two primers,Bnlg1496 and umc1222,which showed mutually supplementary bands of their parents and could identify the Jindan999＇s parents and hybrid clearly,stably and exactly,were screened out by SSR marker from 30 pairs of SSR primers to identify the purity of Jindan999 hybrid maize seed rapidly and exactly,effectively prevent the circulation of fake seeds on the market and enhance seed quality,and provide reference for purity identification of other hybrid maize seed.The results showed that Bnlg1496 and umc1222 could identify the purity of Jindan999 hybrid maize seed completely and its purity was 98.12%.