基于血浆DNA检测妊娠山羊的胚胎性别研究

Embryo Sex Identification of Pregnancy Goats by Determining Plasma DNA

为快速进行山羊无创伤产前的性别诊断,同时对家畜的遗传缺陷检测和胚胎移植等研究提供科学依据,采用改良酚/氯仿法提取妊娠母羊血浆DNA,通过双重PCR技术扩增雄性性别决定(sex deter-mining region Y,SRY)基因,并以真核生物延伸生长因-子1(elongation factor 1 alpha,EF-1α)基因为内参照进行试验。结果显示,15例孕有雄性胎儿的孕母羊血浆样品中,13例扩增出SRY基因片段,检出率为86.67%(13/15);孕有雌性胎儿的孕母羊血浆中均未检出SRY基因片段,雌性和雄性检测的正确率为92.86%(26/28)。

The SPY gene in plasma DNA extracted from pregnancy goats was amplified by taking the elongation factor 1 alpha（EF-1α） as CK to determine fetal sex rapidly and to provide the scientific basis for genetic hereditary defect detection and embryo transfer in livestock.The results showed that the SRY gene fragment in plasma DNA of 13 pregnancy goats was amplified in 15 pregnancy goats with male fetus and its detection rate was up to 86.67%（13/15）,the SRY gene fragment was not detected from plasma DNA of pregnancy goats with female fetus,and the accuracy rate of detecting female and male fetus was 92.86%（26/28）.