天然水体有毒有机污染物毒性监测样品前处理

Sample Pretreatment for Toxic Assessment of Organic Pollutants in River Water 

应用水样直接测试、ＣＨ２Ｃｌ２ 萃取和被动式半渗透膜采样技术（ｔｒｉｏｌｅｉｎＳＰＭＤ）富集洋河宣化至官厅水库沿线水样中污染物，并进行Ｐ．Ｐｈｏｓｐｈｏｒｅｕｍ Ｔ３ 和Ｖｉｂｒｉｏｑｉｎｇｈａｉｅｎｓｉｓｓｐ． ＮｏｖＱ６７ 发光菌急性毒性测试，分析探讨不同样品前处理方式对毒性测试结果的影响．结果显示由原河水样品直接进行的发光菌急性毒性测试会受水体中共存物质的干扰，将毒性掩盖，表现为刺激发光．采用ＣＨ２Ｃｌ２ 萃取样品和ｔｒｉｏｌｅｉｎＳＰＭＤ富集样品后再进行毒性检验，宣化段和下游河段存在有机有毒污染物的毒性．用ｔｒｉｏｌｅｉｎＳＰＭＤ富集的样品，２ 种发光菌检验结果有较好的相关性（ＥＣ５０，ｔ３＝ １．４７ ＥＣ５０，Ｑ６７＋ ６３．１６，ｒ２＝ ０．９８４８）．本文结合ｔｒｉｏｌｅｉｎＳＰＭＤ的优点。

The aim of this research is to verify influences of sample pretreatment on toxic assessment of organic pollutants.Luminescent bacteria P.phosphoreum T3 and Virbrio qinghaiensis sp. Nov Q67 were applied to assay the acute toxicity and river water pretreated with either liquid liquid extraction using dichloromethane or sequestration by a triolein filled semi permeable membrane device (triolein SPMD).The results indicated clearly that substances that lead to stimulating effects influenced the toxic assay performed on original water. After extraction with CH 2Cl 2 or preconcentration with triolein SPMD,acute toxicity of organic pollutants in river water can be monitored.When triolein SPMD was applied,both toxic assays by P.phosphoreum T3 and Vibrio qinghaiensis sp.Nov Q67 gave an accordant result (EC 50,t3 =1.47 EC 50,Q67 +63.16, r 2=0.9848).Based on the comparison,triolein SPMD was recommended for sequestration of organic pollutants from river water for toxic assessment.