ABCG2在不同微环境下肾上腺皮质癌细胞中的表达及意义

THE SIGNIFICANCE OF ABCG2 EXPRESSION ON ADRENOCORTICAL CARCINOMA IN VARIOUS MICROENVIRONMENT

目的:探讨ABCG2在不同微环境下在肾上腺皮质癌细胞中的表达和意义。方法:利用SW-13细胞株建立体内及体外微环境模型,流式细胞技术检测ABCG2在不同微环境下肾上腺皮质癌组织及细胞中的表达情况。结果:ABCG2在裸鼠转移淋巴结中的表达率[(5.41±2.57)%]明显高于标准培养基[(1.04±1.01)%]、干细胞培养基[(2.44±2.30)%]、裸鼠皮下移植瘤[(0.89±1.43)%]及腹腔转移瘤[(3.14±2.42)%]微环境的表达率,差异有统计学意义(P<0.05)。结论:ABCG2在不同微环境下肾上腺皮质癌细胞中的表达有差异,ABCG2为骨髓侧群细胞(side population,SP)特异表型,肾上腺皮质癌组织中可能存在SP细胞。

Objective：To investigate the ABCG2 expression on adrenocortical carcinoma in various microenvironment.Methods：Adrenocortical carcinoma cell line（SW-13） cells were used to established different microenvironment in vitro and in vivo,the tissue and cells of above mention were detected with flow cytometry.Results：The expression of ABCG2 on SW-13 cells in metastasis lymph [（5.41±2.57）%] was higher than that in standard culture medium [（1.04±1.01）%];in stem cell culture environment [（2.44±2.30）%];in subcutaneous transplanted tumor[（0.89±1.43）%] and in peritoneal metastasis tumor[（3.14±2.42）%],which showed a significant difference（P0.05）.Conclusion：The expression of ABCG2 is different in various microenvironment.The phenotype ABCG2 considered to be a specific marker of side population cells（SP）.Adrenocortical carcinoma maybe contain SP cells,as many cancer.