春兰GLO基因的克隆和实时定量表达分析

Cloning and Real-time Expression Analysis of GLO Gene from Cymbidium goeringii

采用RT-PCR结合RACE技术从春兰(Cymbidium goeringii)中分离到一个GLO基因。该基因含有一个630 bp的开放阅读框(ORF),共编码210个氨基酸。系统进化树分析显示,该基因属于B类MADS-box基因的PI/GLO家族,其编码的蛋白与其他植物PI/GLO类蛋白具有很高的同源性,命名为CgGLO(登录号HM106984)。实时荧光定量表达分析表明,CgGLO主要在第二轮花器官唇瓣和花瓣中表达,在萼片、子房和叶片中表达较少,在蕊柱和根中表达量最少,这种表达模式支持了van Tunen对ABC模型的修正,也显示了CgGLO基因可能在春兰花器官以及子房的形成过程中起着重要作用。

In this study,a novel GLO gene was isolated from Cymbidium goeringii by RT-PCR and RACE-PCR techniques.Sequence analysis showed the gene contained an open reading frame of 630 bp encoding a putative protein of 210 amino acids.Homology analysis showed that the deduced protein showed high identity with other PI/GLO proteins from different species,named CgGLO（GenBank accession number HM106984）.Phylogenetic tree analysis also indicated that the B-functional gene CgGLO belongs to PI/GLO clade of MADS-box gene family.Real-time quantitative PCR demonstrated that CgGLO was strongly expressed in 2 whorls organ,i.e.lips and petals,much lower in ovary,leaves and sepals,and lowest in root and columns.It supported the modified ABC model by van Tunen.These results also displayed that CgGLO might regulate the floral organ identity and ovary development of C.goeringii.