药用植物虎杖和盾叶薯蓣的组织培养

Tissue Culture of Reynoutria japonica and Dioscorea zingiberensi

以虎杖和盾叶薯蓣嫩芽为外植体,研究不同的消毒方法和激素水平对组织培养的影响,并筛选合适的培养基配方.结果表明,在初代培养中,虎杖和盾叶薯蓣嫩芽消毒均以0.1%升汞处理10 min为宜;虎杖嫩芽愈伤组织诱导的适宜培养基为MS+6-BA 1.0 mg/L+2,4-D 0.2 mg/L,该愈伤组织在原培养基中经弱光照射后,再经过8周即分化出茎和叶.盾叶薯蓣愈伤组织诱导的适宜培养基为MS+6-BA 2.0mg/L+2,4-D 0.5 mg/L,茎叶分化培养基为MS+6-BA 0.1 mg/L+2,4-D 0.5 mg/L.pH值均为5.8.

Taking buds of Polygonum cuspidatum and Dioscorea zingiberensi as explants in tissue culture,the effects of different sterilization methods and hormonal levels were studied,and their optimal induction medium were researched.According to experiments,the best disinfectant method for their bud was dip in 0.1% HgCl2 for ten minutes in their primary culture.The optimal induction medium for callus formation of Polygonum cuspidatum was MS＋6-BA 1.0 mg/L＋2,4-D 0.2 mg/L.These calluses differentiated stems and leaves after 8 weeks in the same medium when they were cultured in weak light.The optimal induction medium for callus formation of Dioscorea zingiberensi was MS＋6-BA 2.0 mg/L＋2,4-D 0.5 mg/L and differentiating stem and leaf medium was MS＋6-BA 0.1 mg/L＋2,4-D 0.5 mg/L.Their pH values were all 5.8.