高脂血症LDL亚类与血脂关系的研究

Relationship between plasma LDL subclass of hyperlipidemia and blood lipids

目的研究健康成人和高脂血症患者LDL各亚类组成,并进一步分析LDL亚类、血脂水平与动脉粥样硬化、冠心病之间的相关性。方法通过非变性梯度聚丙烯酰胺凝胶电泳—免疫印迹法分析了87例高脂血症患者及81例正常人血清LDL各亚类含量,分布以及平均直径。结果与对照组相比,高脂血症患者血清TC、TG、LDL-C、apoB100,sdLDL水平均有显著升高;HDL-C、apoAI水平及LDL平均直径均有显著降低。两组女性HDL-C均显著高于男性,而LDL-C则显著低于男性。女性高脂血症患者及对照组女性的sdLDL均显著低于同组男性。高甘油三酯血症患者TG及sdLDL水平均显著高于高胆固醇血症患者,TC、LDL-C水平及LDL平均直径均显著低于高胆固醇血症患者。结论性别差异和血脂水平对LDL亚类分布有显著影响。血清TG水平升高作为sdLDL含量升高的主要危险因素,在AS和CHD的发生发展中起主要作用。血浆sdLDL亚类的检测对AS和CHD的早期诊断有积极意义。

Objective To study on the distribution of plasma LDL subclasses in healthy subjects and hyperlipidemia subjects,and investigate the relationships between lipoproteins and LDL subclasses distribution.Methods The distribution of serum LDL subclasses in 87 hyperlipidemia subjects（male 49,female 38） and 81 healthy subjects（male 46,female 35） was detected with non-denaturing gradient polyacrylamide gel electrophoresis and Western blot.Results Gender difference and lipid level had significant influence on LDL subclasses distribution.Conclusion The increase of serum TG level could be a risky factor of the increase of sdLDL level.