简单快速测定酵母的絮凝性

A Quick and Simple Way for Determining the Flocculence of Yeast Cell

介绍一种利用细胞表面疏水性原理快速而简单测定酵母细胞絮凝性的方法(HICF法)。酵母细胞悬浮液注入Sepharose凝胶柱 ,然后加入含有氯化钠的缓冲液 ,疏水细胞吸附于凝胶柱 ,通过测定吸附于凝胶柱的细胞的百分数可反映出酵母细胞的疏水程度[1],以此指示酵母的絮凝性。

This article introduced a quick and simple way for determining the flocculence of yeast cell(CHICF)using the principle of the hydrophobicity on the surface of cell.The yeast cells suspension was injected into sepharose coagel column,then added with the sodium chloride buffer solution,the hydrophobic cells were absorbed on coagel column.After the above operations,The hydrophobic level of yeast cells which indicated the flocculence of yeast cell was reflected by percentage of cells on the coagel colunm.