定量检测大内皮素-1对心血管疾病的诊断价值

The clinical value of big endothelin-1 quantitative detection in diagnosis of cardiovascular diseases

目的评价血浆大内皮素-1(BigET-1)定量检测对心血管疾病患者的诊断价值。方法采用定量ELISA改进法检测不同心血管疾病患者的血浆BigET-1含量。结果心血管疾病患者的血浆BigET-1水平明显增高,并随心血管疾病程度的加重而呈线性增加;实验受试者工作特征曲线下面积为0.931;心血管疾病的诊断敏感性和特异性分别为92.5%和96.5%。结论定量检测血浆BigET-1是目前用于评价心血管疾病患者的最佳实验手段。

Objective To evaluate the diagnostic value of big endothelin-1（Big ET-1） quantitative detection in patients with cardiovascular diseases.Methods Quantitative enzyme-linked immunosorbent assay（ELISA）improvement method was applied to measuring plasma level of Big ET-1 in patients with different degree of cardiovascular diseases.Results The plasma level of Big ET-1 was significantly elevated in patients with cardiovascular diseases.With the increase of extent of cardiovascular diseases,Big ET-1 increased in line.Receiver operator characteristic curve（ROC） showed that area under curve（AUC） was 0.931.The sensitivity and specificity were 92.5% and 96.5% respectively for diagnosis of cardiovascular diseases.Conclusion Quantitative detection of Big ET-1 is the best laboratory test at present for evaluating extent of patients with cardiovascular diseases.