新疆野苹果天然群体抗寒性的电阻抗图谱分析

Assessing Frost Hardiness of Malus sieversii Natural Populations by Electrical Impedance Spectroscopy

以新疆巩留县和新源县2个居群下的7个新疆野苹果群体当年生枝条为材料,应用EIS法对各群体抗寒性进行测定,并与电导率法进行对比分析。结果表明:巩留和新源2个居群内群体间的半致死温度差异不显著,2个居群间的差异显著,巩留居群的半致死温度显著低于新源居群,证明巩留居群的抗寒性高于新源居群。电阻抗图谱法单-DCE模型中6个参数(τ,ψ,r,r1,re,ri)中,r1,re,τ3个参数可以得出供试样品的半致死温度,其中r1,re所得的半致死温度与电导率法相关系数达到极显著水平,说明参数r1,re适用于新疆野苹果的抗寒性测定。电阻抗图谱法(EIS)与电导率法的抗寒性测定结果基本一致,但省时、不需温育,具有更大优越性。

Malus sieversii is an important germplasm resource,and valuable for genetic improvement and conservation of genetic diversity of apples.In this paper,one-year-old seedlings from 7 populations of Malus sieversii in Gongliu and Xinyuan of Xinjiang were used.Cold hardiness of the seedlings was determined by means of the EIS method in comparison with EL method.The results with different cold hardiness determination methods indicated that there was no obvious difference in the semilethal temperature within populations of Gongliu and Xinyuan.In contrast,there was significant difference in the semilethal temperature between the two populations.The semilethal temperature of Gongliu populations was significantly lower than that of Xinyuan populations.Thus,the cold resistance of Gongliu populations was higher than that of Xinyuan populations.Among the six parameters （τ,ψ,r,r1,re and ri） in the Single DEC model of electrical impedance spectroscopy,was obtained by three parameters （r1,re and τ） was able to be used t determine semilethal temperature of the tested samples.The semilethal temperature obtained by r1 and re was extremely significantly correlated with the conductivity method,suggesting that r1 and re could be used to determine the M.sieversii cold resistance.The cold hardiness estimated by the EIS method was basically similar to that,assessed by means of the traditional EL method.The EIS method is superior over the EL owing to its time saving and no incubation.