河南宝丰气象因子与烤烟DNA甲基化关系分析

Study of Relationship Between DNA Methylation of Flue-cured Tobacco and Meteorological Factors in Baofen

通过建立宝丰气象因子与烤烟烟叶DNA甲基化的数学模型,找出影响宝丰烟叶DNA甲基化的主要气象因子,为研究宝丰烟叶品质和风格的形成提供一种方法。以灰色关联分析筛选出对DNA甲基化影响大的4个气象因子为自变量,把DNA甲基化作为因变量,建立修正的Logistic数学模型并进行分析。结果表明,单因子对DNA甲基化影响程度依次为平均最小湿度、平均日照时数、平均气温差,且为正相关;总降水量与DNA甲基化水平呈负相关。两因素交互效应分析表明,平均最小湿度、平均气温差、平均日照时数之间对DNA甲基化水平均具有协同效应,总降水量与平均最小湿度和平均气温差之间对DNA甲基化的影响有拮抗作用。

This study was conducted to build the model for explaining the relationship between the meteorological factors and DNA methylation of flue-cured tobacco.These results provide a method for the formation of the style and characteristic of tobacco.Four meteorological factors were screened with comprehensive evaluation based on grey relational analysis.Then the revised Logistic model was built between DNA methylation and four factors.The results of single-factor analysis showed that the average temperature difference（ATD）,average sunshine hour（ASH）,and mean relative humidity（MRH） were positively correlated with DNA methylation.The order of the three factors effecting the DNA methylation was as follows：MRH ASH ATD.Total precipitation（TP） was negatively correlated with DNA methylation.Two-factor interactions analysis indicated that the synergistic effects between ATD and ASH/MRH or between ASH and MRH were shown in an interaction range,respectively.TP and MRH or ATD have the antagonistic effects with DNA methylation.