干化学法检测氯离子的实验研究

Experimental study on the detection of chloridion based on dry chemistry method

目的 研究干化学试剂和微型光谱仪技术快速检测氯离子的方法.方法 将所配制的电解质氯离子液体试剂冻干于微型光谱仪配套的检测杯中,运用冷冻干燥机真空抽干,制备成干粉状试剂,封存备用.测定时,在配制好的干试剂检测杯中加入标本和稀释液后,放入微型光谱仪的检测卡槽中,经微型光谱仪的磁力搅拌系统混匀,经加热系统孵育后,运用速率法的反应原理,于405 nm处进行氯离子的吸光度（A）值检测.并对试剂的性能进行评价,将结果进行统计学分析.结果 该指标线性范围为40～160 mmol/L,冻干后90 d内反应稳定性良好.其批内变异系数（CV）〈4%,批间CV〈5%,回收率为96.5%～104.4%.当血清内胆红素（TB）〈280.0 μmol/L以及三酰甘油（TG）〈12.0 mmol/L时,结果未见明显干扰.结果显示,与全自动生化分析仪具有良好的相关性（r=0.976 9）.结论 以微型光谱仪干试剂法测定电解质氯离子结果准确可靠,检测过程简便,可用眼直接观察颜色变化,避免漏检且仪器试剂携带方便,符合战地救治、基层医疗的需要.

Objective To establish a new method for rapid detection of chloridion based on micro spectrometer and dry reagent. Methods The liquid chloridion reagent was lyophilized for the preparation of reagent in powder form,which was then sealed into a detector cup of micro spectrometer for later experiment. During determination, the detector cup,in which the specimens and diluents were added to the dry reagent,was put into the detector slot of micro spectrometer,the contents in the detector cup were then well mixed by the magnetic stirring system of micro spectrometer and incubated with the heating system. Afterwards, the A values of chloridion was detected respectively at 405nm following the reaction principles of turbidimetry method. Based on the findings of those detections mentioned above, the performance of the chloridion dry reagent was evaluated, and the results were then statistical ly analyzed. Results The linear range of chloridion could meet the demand for clinical analysis,and the dry reagents had good reac tion stability for 60 days after being lyophilized, with the intra-assay coefficient variation（CV）less than 4%, inter-assay CV less than 5%, and the recovery rate form96.5 % to104.4 %. No obvious interference was observed in the determination results under the circumstance that the serum TB was less than 280 μmol/L and the TG was less than 12.0 mmol/L. The detection results by this method were well correlated with these of traditional liquid reagent enzymic method（r 0. 976 9）. Conclusion The method for rapid detection of chloridion based on micro spectrometer and dry reagen is easy and reliable,and can meet the clinical practice requirement in basic hospitals and battlefield treat and cure.