摘要
目的 探讨HBeAg阴性的乙型肝炎(HBV)感染者前S1抗原阳性与前C区变异发生频率、HBV DNA阳性率之间的关系.方法 用荧光定量PCR检测313例HBeAg阴性HBV感染者血清HBV DNA载量,ELISA法检测前S1抗原,测序分析HBV前C区nt1 762~nt1 764位和 nt1 896位点基因突变.结果 313例血清HBV DNA阳性175例(55.6%),阴性138(44.1%);175例HBV DNA阳性的标本中有155例(88.6%)前S1抗原阳性,138例HBV DNA阴性的标本中前S1抗原阳性33例(23.9%).HBV DNA与前S1抗原结果比较差异有统计学意义(P〈0.001);总前S1抗原阳性188例,发生nt1 896位变异为160例,nt1 762~nt1 764位变异为117例.前S1抗原阴性的125例中,发生nt1 896位变异为67例,nt1 762~nt1 764位变异为69例.前S1抗原阳性与阴性患者nt 1 896位点变异率比较差异有统计学意义(P〈0.05).结论 HBeAg阴性的HBV感染者中,前S1抗原不受前C区变异影响,可更客观地反映HBV复制状态.
Objective To Analysis of the relationship of serum PreS1,HBV DNA and mutation frequency of Pre C in HBV infection patients with negative HBeAg. Methods PreS1 and HBeAg were detected by ELISA,and HBV-DNA examined by fluorescence quantitative PCR,mutation of PreC nt1 762-nt1 764 and nt1896 were analysis by sequencing. Results Among 313 HBeAg (-)patients,the positive of HBV-DNA(+)was 175(55.6%),HBV-DNA(%)was 138(44.1%). In 175 HBV DNA(+)patients, 155 (88.6% ) patients PreS1 were positive and 33 (23.9 % ) PreS1 (+) in 138 HBV-DNA(-) patients, the difference is significant(P〈0. 001) ;The total positive PreS1 Ag patients were 188,mutation in nt1 896 160 patients and 117 in nt1762-nt1 764,meanwhile,among 125 PreS(-)patients, mutation in nt1896 were 67 patients and 69 in nt1 762-nt1 764. The difference of frequency in ntl 896 mutation between positive and negative PreS1-Ag was statistically significant(P〈0.05). Conclusion In the patients with HBeAg negative,PreS1-Ag is not affected by mutation of Pre C gene and affect the copy of HBV objectively.
出处
《国际检验医学杂志》
CAS
2011年第7期766-767,769,共3页
International Journal of Laboratory Medicine