Campath-1H对食蟹猴免疫细胞的清除作用和免疫重建研究

Campath-1H depletes lymphocytes in blood and lymphatic tissues of cynomolgus monkeys

目的:研究免疫诱导剂Campath-1H对淋巴组织免疫细胞的清除和重建的时效性。方法:筛选雄性成年食蟹猴15只,随机分成五组,给予相同剂量的Campath-1H(3.0 mg/kg),测定Campath-1H的药物代谢参数,并分别在给药前、给药后3、9、14、21和35 d检测外周血、脾、淋巴结等免疫细胞的清除和重建规律。结果:Cam-path-1H对外周血淋巴细胞清除最显著,对单核细胞、NK细胞作用较小,对粒细胞没有清除作用。结论:Cam-path-1H对食蟹猴外周血各类免疫细胞清除效能不同,可能与细胞表面CD52抗原密度有关;对次级淋巴组织淋巴细胞的清除效能和抑制时间低于外周血。

Objective： To investigate the effect of Campath-1H induction therapy on lymphodepletion in cynomolgus monkeys.Methods： Fifteen screened cynomolgus monkeys were randomly enrolled into 5 groups（n=3）,and each of them received a single injection of Campath-1H at the dose of 3.0 mg/kg.Monkeys were anesthesied at each scheduled timepoints,then peripheral blood and secondary lymphatic tissues were obtained for flow cytometric analysis and histological examination.Results： Campath-1H showed different depletional profiles on lymphocytes,monocytes,NK cells and granulocytes.In contrasting with peripheral blood,spleen and lymph node lymphocytes were relatively resistant to Campath-1H-induced depletion.Conclusion： Depletional efficiency of Campath-1H was determined by cell surface expression of CD52 antigen.And biopsy of lymph node is necessary and may be sufficient to assess lymphocytes clearance in secondary lymphoid organs in this model.