一例噬血细胞综合征与人疱疹病毒7型感染相关性的报告

One case of correlation between the human herpesvirus 7 DNA load and the hemo hagocytic syndrome

目的研究人疱疹病毒7型（HHV一7）病毒感染与噬血细胞综合征发生的关系，为临床噬血细胞综合征患者诊断和抗病毒的治疗提供依据。方法收集噬血细胞综合征患者不同治疗期外周血，提取DNA，合成HHV一7引物，PCR扩增片段，基因序列分析．荧光定量PCR法测定病毒载量，化学发光法测定血液铁蛋F_1的浓度，分析二者的相关性。结果经PCR扩增和基因序列分析在噬血细胞综合征外周血中检测到HHV一7基因片段，其HHV．7病毒载量随着血液铁蛋门的浓度而变化。结论HHV一7感染可以继发引起噬血细胞综合征并且与HHV一7病毒载量有关。

Objective To research the relationship between human herpesvirus 7 （HHV-7） viral Load and the etiopathogenisis of hemophagocytic syndrome, in order to provide evidence for the clinical diagnosis of hemophagocytic syndrome and anti-virus therapy. Methods Peripheral blood of patient with hemophagocytic syndrome during different treatment periods, extracted DNA, Syntheticed the primers of HHV-7 ,gene sequence of PCR amplified fragments detected, determined HHV-7 viral Load by Real - time fluorescent quantitative PCR and the ferritin concentration in peripheral blood detected by chemiluminescence. Result The sequence result indicated that PCR amplified fragment was a part of HHV- 7 gene, the ferritin concentration viried with the load of HHV-7. Conclusion The occurrence of hemophagocytic syndrome is connetted with the load of HHV-7.