转录因子Sp8在成年大鼠侧脑室下区新生神经元中的表达

Expression of transcription factor Sp8 in the neuroblasts derived from the adult rat subventricular zone

目的观察转录因子Sp8在成年大鼠侧脑室下区新生神经元中的表达。方法 4只成年大鼠经心脏灌注固定,利用免疫荧光标记法并结合激光共焦显微镜扫描技术,观察Sp8在大鼠侧脑室下区新生神经元中的表达。结果成年大鼠整个侧脑室下区,从吻侧到尾侧都存在大量的新生神经元,这些新生神经元分布多呈链状;与尾侧相比,吻侧侧脑室下区有更多的新生神经元产生。整个侧脑室下区的背外侧区域产生的新生神经元数量最多且分布最密集;而且这些新生神经元中的绝大多数都表达转录因子Sp8。结论 Sp8在侧脑室下区具有广泛的表达,可能与新生神经元的产生及相关的调控过程关系密切。

Objective Investigating the expression of transcription factor specificity proteins 8 （Sp8） in the neuroblasts produced by the neural stem cells in adult rat subventricular zone. Methods Four adult rats were killed through intracardiac perfusion to get the brain samples, and immunofluorescence staining and laser confocal microscope scanning were performed to observe the expression of transcription factor Sp8 in the neuroblasts produced by the neural stem cells in the subventricular zone. Results There were a large number of neuroblasts derived from the neural stem cells in the entire subventricular zone,from rostral to caudal regions. The dorsal-lateral region gave rise to most of the neuroblasts, and these neuroblasts usually distributed intensively as a chain. Compared to the caudal regions, the rostral regions generated more neuroblasts and the vast majority of them expressed the transcription factor Sp8. Conclusion Transcription factor Sp8 is expressed widely in the subventricular zone and might participate in the generation of neuroblasts and interrelate regulation mechanism.