血管内皮生长因子、Eph受体酪氨酸激酶A2、基质金属蛋白酶-2和-9在卵巢癌血管生成拟态中的作用

Effects of vascular endothelial growth factor,Eph receptor A2,matrix metalloproteinase-2 and-9 in ovarian carcinoma vasculogenic mimicry

目的探讨血管内皮生长因子(VEGF)、Eph受体酪氨酸激酶A2(EphA2)、基质金属蛋白酶(MMP)-2和MMP-9在卵巢癌血管生成拟态中的作用。方法收集临床和预后资料完整的卵巢癌组织标本84例,切片经明确诊断后进行CD31和过碘酸-雪夫(PAS)双重染色,证实肿瘤组织中存在血管生成拟态,行VEGF、EphA2、MMP-2和MMP-9免疫组织化学染色,根据染色指数统计免疫组织化学染色结果。结果有血管生成拟态组(36/84)和无血管生成拟态组卵巢癌组织(48/84)的VEGF、EphA2、MMP-9表达差异有统计学意义,有血管生成拟态组的卵巢癌细胞VEGF、EphA2、MMP-9表达明显高于无血管生成拟态组。但有血管生成拟态组和无血管生成拟态组患者的MMP-2表达差异无统计学意义。结论在卵巢癌中血管生成拟态和内皮依赖性血管并存,VEGF、EphA2和MMP-9等参与了卵巢癌血管生成拟态的形成。检测VEGF、EphA2和MMP-9可作为预测卵巢癌预后的间接指标。

Objective To investigate the effect of vascular endothelial growth factor （VEGF）, Eph receptor A2 （EphA2） , matrix metalloproteinases（MMP）-2 and MMP-9 in ovarian carcinoma vasculogenic mimicry（ VM）. Methods Totally 84 ovarian carcinoma specimens with complete clinical and prognostic data were collected, and using CD31 and PAS double staining to confirm if vasculogenic mimicry exists in the ovarian carcinoma specimens. Immunohistochemical method was used to detect VEGF, EphA2, MMP-2 and MMP-9, examining the results of the immunohistochemical method according to the dye index. Results VEGF, EphA2 and MMP-9 in ovarian carcinoma specimens had significant differences between the VM group （36/84） and non-VM group （48/84）, the expression in the VM group showed significantly higher than that in non-VM group. But the expression of MMP-2 between the two groups was not statistically significant. Conclusion Vasculogenic mimicry and endothelium-derived angiogenesis are co-existed in ovarian carcinoma, VEGF, EphA2 and MMP-9 play important roles in vasculogenic mimicry. Detecting VEGF, EphA2 and MMP-9 can be used to evaluate the prognosis of ovarian carainoma.