木瓜药材HPLC指纹图谱研究

Study on HPLC Finger Print of Chinese Quince

以熊果酸为参照物,利用高效液相色谱(HPLC)梯度洗脱,测定了19批木瓜(Chaenomeles speciosa(Sweet)Nakai)样品。建立了药用木瓜的高效液相指纹图谱,为评价控制药用木瓜的质量提供了依据。色谱柱为YPW-Kromasil TM-C18柱(250 mm×4.6 mm,5μm)(美国迪马公司);流动相:甲醇(A)-1%冰醋酸(B),流动相A为甲醇,流动相B为1%冰醋酸水溶液。检测波长290 nm,柱温30℃,流速1.0 mL/min,进样量20μL。通过分析19批木瓜样品得到的高效液相指纹图谱有11个共有峰,多数峰都可以达到较好分离且19批次相似度符合。因此药用木瓜的指纹图谱特征性及专属性强,可用于药用木瓜的质量控制。

The chromatogram fingerprint of the compound Chaenomeles speciosa （Sweet） Nakai was established by HPLC. Analysis was performed on a YPW-Kromasil TM -C18 analytical column （250 mm x 4.6 mm, 5 μm） with methanol （A） and 1% glacial acetic acid （B） as mobile phases in gradient mode. The column temperature was set at 30℃ and the flow rate was 1.0 mL/min with the detection wavelength of 290 nm. By analyzing the fingerprint chromatogram,Chaenomeles speciosa showed 11 characteristic peaks established from 19 specimens. The similarity of each specimen was good. The accuracy, repeatability, and stability of this method were satisfied,the RSDs of relative retention time and area of aimed peaks existed in all samples. Results showed that the chromatographic fingerprint of Chaenomeles speciosa with high characteristics and specificity can be used as theories dependence to control its quality.