电针足三里穴对不完全性肠梗阻大鼠小肠肌电活动的影响

Effect of electroacupuncture at Tsusanli on intestinal myoelectric activity in rats with incomplete intestinal obstruction

目的:探讨电针足三里穴对肠梗阻大鼠小肠肌电活动的影响.方法:采用非贯穿肠管的方式,末端回肠套环建立不完全性肠梗阻大鼠模型,将大鼠随机分为:空白对照组(n=10)、假手术组(n=10)、肠梗阻组(IO组,n=10)、肠梗阻+电针组(14dIO+EA组,n=10,21dIO+EA组,n=10).造模成功后空白对照组、假手术组、IO组均未给予电针治疗措施,IO+EA组连续给予电针14d、21d电针治疗措施.最后1次电针后2h,分别测体质量后打开腹腔,肉眼观察回肠组织形态学的改变,BL-420F生物机能实验系统测定回肠肌电.结果:IO组大鼠体质量较空白对照组和假手术组显著降低(P<0.01),IO+EA组大鼠体质量较IO组显著升高(P<0.01).回肠肌电慢波活动改变情况:14dIO组振幅(mV)低于空白对照组(0.11±0.03vs0.35±0.06,P<0.01),且频率(%)、振幅(%)变异系数均明显高于空白对照组和假手术组(27.71±10.54vs14.08±4.22,22.00±6.24;75.54±8.59vs15.84±1.49,20.67±7.57,均P<0.01);电针实验治疗IO+EA组大鼠14d后,梗阻段平均频率(次/min)均较IO组增加(33.18±2.56vs24.01±0.92,P<0.01);平均振幅(mV)增大(0.25±0.09vs0.11±0.03,P<0.05);频率变异系数(%)减少(17.02±3.62vs27.71±10.54,P<0.05);振幅变异系数(%)减少(58.39±9.56vs75.54±8.59,P<0.01);电针实验治疗21d后,梗阻段平均频率(次/min)均较IO组增加(40.55±5.29vs33.18±2.56,P<0.01),振幅变异系数(%)减少(44.00±11.61vs58.39±9.56,P<0.05).结论:采用非贯穿肠管的方式能有效建立不完全性小肠梗阻模型;电针足三里穴通过调节肠肌电,对大鼠不完全性肠梗阻实验性治疗有一定的效果,在一定程度上对肠梗阻大鼠的胃肠动力有较好的调节作用.

AIM： To investigate the effect of electroacupuncture at Tsusanli on the regulation of gastrointestinal motility in rats with incomplete intestinal obstruction.METHODS： The 1/2 terminal ileum of rats with intestinal obstruction was ligated to generate a model of incomplete intestinal obstruction. Rats were randomly divided into control group （n = 10）, sham-operated group （n = 10）, intestinal obstruction group （IO group, n = 10）, and intestinal obstruction ＋ electroacupuncture group （14 d IO ＋ EA subgroup, n = 10, 21 d IO ＋ EA subgroup, n = 10）. At 2 hours after the last treatment, changes in body weight and myoelectric slow wave activity in the ileum were recorded, and ileal histomorphological changes were observed with the unaided eyes.RESULTS： Compared to the control group, rats of the IO group showed signifi cant weight loss （P 0.01）. However, the body weight increased obviously in the IO＋EA group compared to the IO group （P 0.01）. The amplitude （mV） for the myoelectric slow wave in the IO group on day 14 was significantly lower than that in the control group （0.11 ± 0.03 vs 0.35 ± 0.06, P 0.01）, and the coeffi cient of variation （CV） of the mean frequency （%） and amplitude （%） was signifi cantly higher in the IO group than in the control and sham-operated groups （27.71 ± 10.54 vs 14.08 ± 4.22, 22.00 ± 6.24; 75.54 ± 8.59 vs 15.84 ± 1.49, 20.67 ± 7.57, all P 0.01）. After 14 days of electroacupuncture, the mean frequency for the myoelectric slow wave in the ileum in the IO ＋ EA group was significantly higher than that in the IO group （33.18 ± 2.56 vs 24.01 ± 0.92, P 0.01）. Compared to the IO group, the mean amplitude （mV） in the IO ＋ EA group remarkably increased on day 5 （0.25 ± 0.09 vs 0.11 ± 0.03, P 0.05）. The CV of frequency （%） in the IO ＋ EA group was significantly lower than that in the IO group （17.02 ± 3.62 vs 27.71 ± 10.54, P 0.05）. The CV of amplitude （%） in the IO ＋ EA group was signif icantly lower than that in the IO group （58.39 ± 9.56 vs 75.54 ± 8.59, P 0.01）. After 21 days of electroacupuncture, the mean frequency in the ileum in the IO ＋ EA group signif icantly increased compared to the IO group （40.55 ± 5.29vs 33.18 ± 2.56, P 0.01）. Compared to the IO group, the CV of amplitude （%） in the IO ＋ EA group was signifi cantly lower （44.00 ± 11.61 vs 58.39 ± 9.56, P 0.05）.CONCLUSION： A rat model of incomplete intestinal obstruction could be generated by ligating the 1/2 terminal ileum of rats with intestinal obstruction. Electroacupuncture at Tsusanli exerts a therapeutic effect against incomplete intestinal obstruction by regulating intestinal myoelectric activity.