急慢性大鼠梗阻性黄疸模型的建立

Evaluation of rat models of acute and chronic obstructive jaundice

目的:探讨急慢性SD大鼠梗阻性黄疸模型的建立,评价其模拟临床梗阻性黄疸的价值,提供一种新型的慢性梗阻性黄疸模型.方法:SD大鼠随机分成3组,每组每时段6只:急性梗阻性黄疸模型组(A组),慢性梗阻性黄疸模型组(B组),对照组(C组).造模后于第1、2、3、4、5周分别抽取大鼠静脉血检查肝功能,显微镜下刻度目镜检测大鼠胆总管直径,光学显微镜观察肝组织的病理改变,其中第4周行胆总管十二指肠吻合再通.结果:A组造模术后4wk内黄疸进行性加重(TB:749.38μmol/L±38.99μmol/Lvs84.86μmol/L±49.09μmol/L,P<0.05),胆总管直径扩张明显(1.50cm±0.30cmvs0.35cm±0.15cm,P<0.05),肝细胞变性、坏死明显,伴有增生.第4周行胆总管与十二指肠端侧吻合后黄疸消退明显(TB:153.93μmol/L±57.36μmol/Lvs749.38μmol/L±38.99μmol/L).B组造模术后黄疸呈现波动性,胆总管直径扩张(0.20cm±0.15cmvs0.30cm±0.10cm,P<0.05),肝细胞以变性为主,并有纤维组织增生.第4周行胆总管与十二指肠端侧吻合再通后黄疸缓慢消退(TB:32.15μmol/L±13.20μmol/Lvs36.52μmol/L±16.28μmol/L,P<0.05).A组各时段的总胆红素及胆总管直径均存在明显不同.结论:慢性大鼠梗阻性黄疸的模型和急性大鼠梗阻性黄疸大鼠有明显不同的表现,可以提供一种理想的慢性梗阻性黄疸的动物模型.

AIM： To develop rat models of acute and chronicobstructive jaundice and to evaluate the extentto which they mimic features of clinical diseases.METHODS： Ninety Sprague-Dawley rats wererandomly and equally divided into three groups：acute obstructive jaundice group, chronic obstructive jaundice group, and control group. Each group was further divided into five subgroups for testing at different time points. Liver function was determined and the diameter of the common bile duct was measured under a microscope at weeks 1, 2, 3, 4, and 5 after model induction. Pathological changes in liver tissue were also observed by microscope. Choledochoduodenostomy was performed at week 4.RESULTS： Jaundice progressively worsened inrats with acute obstructive jaundice, and TBILincreased from 84.86 μmol/L ± 49.09 μmol/L atweek 1 to 749.38 μmol/L ± 38.99 μmol/L at week4. Meanwhile, the diameter of the common bileduct diameter increased from 0.35 cm ± 0.15 cmto 1.50 cm ± 0.30 cm, and obvious degeneration,necrosis and hyperplasia of liver cells were observed. Jaundice was obviously improved after choledochoduodenostomy （TBIL： 153.93 μmol/L ± 57.36 μmol/L; diameter of the common bile duct： 0.40 cm ± 0.20 cm）. Jaundice was mild in rats with chronic obstructive jaundice, and TBIL decreased from 42.43 μmol/L ± 23.56 μmol/L at week 1 to 36.52 μmol/L ± 16.28 μmol/L at week 4. Meanwhile, the diameter of the common bile duct increased from 0.20 cm ± 0.15 cm to 0.30 cm ± 0.10 cm, and obvious degeneration and hyperplasia of liver cells were noted. Jaundice slowly subsided after choledochoduodenostomy （TBIL： 32.15 μmol/L ± 13.20 μmol/L; diameter of the common bile duct： 0.15 cm ± 0.10 cm）. Total bilirubin and the diameter of the common bile duct differed significantly at different time points between rats with acute and chronic obstructive jaundice （both P 0.05）.CONCLUSION： Rat models of acute and chronic obstructive jaundice have been successfully established. There were significantly different manifestations between rats with chronic and acute obstructive jaundice..