EGFL7基因过度表达与喉癌分化、转移及预后的关系

Association of EGFL7 mRNA Overexpression with Differentiation,Metastasis and Prognosis of Laryngocarcinoma

目的探讨喉癌组织中EGFL7基因mRNA表达与喉癌分化、转移及临床预后的关系。方法收集2008年5—11月共42例行喉癌手术患者的切除标本。用免疫组织化学法检测EGFL7蛋白在42例喉癌组织和配对癌旁组织中的表达情况,提取肿瘤及癌旁组织配对标本的总RNA,用逆转录(RT)-PCR方法测定EGFL7基因表达,蛋白质印迹法测定EGFL7蛋白的表达,并结合临床资料,对EGFL7基因差异表达与喉癌患者临床表现的相关性进行分析研究。结果对42例配对标本分别进行EGFL7mRNA荧光检测比较,30例标本的肿瘤组织中EGFL7基因mRNA表达明显高于癌旁正常喉组织。22例标本的肿瘤组织中EGFL7蛋白表达明显高于癌旁正常喉组织。EGFL7mRNA的表达与喉癌淋巴结转移和浸润深度密切相关(P<0.05),而与患者的性别、年龄、吸烟、肿瘤分化程度等无关(P>0.05)。结论 EGFL7基因在喉癌组织中表达状态与喉癌的生长和浸润转移关系密切,EGFL7基因可望作为喉癌病情发展及指导临床治疗的标记物之一。

Objective To investigate the association of EGF-like-domain,multiple 7（EGFL7） mRNA expression level with the differentiation,metastasis and prognosis of laryngocarcinoma.Methods Tissue specimens were obtained from 42 patients undergoing surgery for laryngocarcinoma between May and November,2008.Immunohistochemistry was used to detect the level of EGFL7 in the 42 tumor tissues and matched adjacent normal tissues.Reverse transcriptional PCR（RT-PCR） was performed for amplification of EGFL7 mRNA from the 42 tumor tissues and matched adjacent normal tissues,and westerblot was adopted to determine EGFL7 protein expression.The differential EGFL7 mRNA expression was analyzed for its association with the clinical manifestations of the patients.Results EGFL7 mRNA expression was detected in all the laryngocarcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method.EGFL7 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues in 30 cases,and EGFL7 protein expression was also significantly higher in the tumor tissues than in the adjacent normal laryngeal tissues in 22 cases.Expression of EGFL7 mRNA was highly correlated with lymph node metastasis and T classification（P0.05）,but was not correlated with the patients＇ gender,age,or tumor differentiation（P0.05）.Conclusions EGFL7 mRNA expression is correlated closely with the differentiation and metastasis of laryngocarcinoma.EGFL7 may serve as a marker for assessing the progression of laryngocarcinoma and provide assistance for clinical therapeutic decisions.