摘要
In order to study osmotic adjustment and accumulation of anions and cations in rice (Oryza sativa L.) seedlings under NaCl stress, a greenhouse experiment was conducted using two rice cultivars including IR651 (tolerant) and IR29 (sensitive). Seedlings were grown in Youshida nutrient solution. Salinity treatments were imposed 21 days after sowing with 0 and 100 mM NaCI and seedlings were harvested 0, 72, 120 and 240 hour after salinization. Water (ψw) and osmotic (ψs) potentials, total soluble sugars and inorganic ions (Na+. K+, Cl-, Ca2+ and Mg2+) concentrations and osmotic adjustment were determined in sixth (youngest) leaf. Salinity caused a substantial biomass reduction in rice seedlings, ψs reduction in IR29 was occurred more than IR651. Water potential decreased in both the cultivars under stress conditions, but IR651 was able to maintain higher ψw and kept better growth till the end of the experiment. Osmotic adjustment was observed in IR651 was about 10 times more than in IR29. K+ accumulation decreased in both cultivars under stress condition while Na+ accumulation increased in both the cultivars with higher increase in IR29 seedlings. CI concentration increased in youngest leaf of both IR29 and IR651. Our results showed that tolerant cultivar was able to make osmotic adjustment faster than the sensitive cultivar using high accumulation of solutes especially total soluble sugars.