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硫化氢对大鼠心房肌细胞三磷酸腺苷敏感性钾通道电流的影响 被引量：1

Effects of hydrogen sulfide on the ATP-sensitive K channels current in isolated rat atrial myocytes
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摘要 目的观察内源性及外源性硫化氢(H2S)对大鼠离体心房肌细胞三磷酸腺苷(ATP)敏感性钾通道(KATP)外向电流的影响,以探讨H2S对心房肌细胞的作用。方法对大鼠离体心脏采用胶原酶酶解法得到单个心房肌细胞,采用膜片钳全细胞技术记录H2S生成酶——胱硫醚-γ-裂解酶的不可逆抑制剂DL-propargylglycine(PPG)用药前、用药后5,10,15,20,25 min及不同浓度外源性H2S的供体硫氢化钠(NaHS)干预前后的KATP电流。结果经200μmol/L PPG干预后KATP峰电流密度(+70 mV)显著减小(6.906 6±1.902 9 pA/pF vs 3.924 4±0.988 5 pA/pF,P<0.01),且具有时间依赖性。经9.375,18.75,37.5,75,150μmol/L NaHS干预后KATP峰电流密度呈浓度依赖性增大,至150μmol/L时峰电流密度明显增大(6.5974±1.1527 pA/pF vs 10.463 1±2.329 7 pA/pF,P<0.01)。结论内源性及外源性H2S均可以开放大鼠离体心房肌KATP通道,使KATP电流增加。 Objective To investigate the effects of endogenous and exogenous hydrogen sulfide（ H2S） or, the ATP-sensitive K channels（ KATP ） current in the rat atrial myocytes in vitro. Methods Atrial myocytes were isolated from rat heart using modified Langendoff perfusion and collagenase. KAyP current of single rat atrial myocytes were recorded by whole-cell patch-clamp technique. Result The density of KATP current was decreased by PPG（200 μmol/L） （6. 906 6 ± 1. 902 9 pA/pF vs 3. 924 4±0. 988 5 pA/pF,P 〈 0. 01 ） in a time-dependent way. The density of KATP current was enhanced by NariS（9. 375,18.75,37.5,75,150 txmol/L） in a concentration-dependent manner, and it was obviously increased in the NariS at a concentration of 150 umol/L （6. 597 4 ± 1. 152 7 pA/pF vs 10.463 1± 2. 329 7 pA/pF,P 〈 0.01 ）. Conclusion Endogenous and exogenous H2 S can open KATp channels and enhance the KATP current in rat atrial myocytes.
作者 李艳兵 钟光珍 杨新春 刘秀兰
机构地区 首都医科大学附属北京朝阳医院心脏中心
出处 《中国心脏起搏与心电生理杂志》 北大核心 2011年第3期245-248,共4页 Chinese Journal of Cardiac Pacing and Electrophysiology
基金 国家自然科学基金资助项目(项目编号:30770875) 北京市自然科学基金资助项目(项目编号:7072027)
关键词 心血管病学 硫化氢 三磷酸腺苷敏感性钾通道 膜片钳 心房 Cardiology Hydrogen sulfide ATP-sensitive K channels Patch-clamp technique Atrial
分类号 R331.38 [医药卫生—人体生理学]
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参考文献11

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同被引文献27

  • 1罗兴林,何川,杨艳,李秀琴.血管内皮源性舒张因子对血管平滑肌钙激活钾通道活性影响的对比研究[J].中国实用内科杂志,2005,25(2):134-136. 被引量：1
  • 2Geng B, Yang J, Qi Y,et al. H2 S generated by heart in rat and its effectson cardiac function [ J ]. Biochem Biophys Res Commun, 2004, 313 : 362.
  • 3Zhao W, Zhang J, Lu Y, et al. The vasorelaxant effect of H2 S as anovel endogenous gaseous KATP channel opener [ J ]. EMBO J, 2001,20:6 008.
  • 4Cheng Y, Ndisang JF, Tang G, et al. Hydrogen sulfide inducedre- laxation of resistance mesenteric artery beds of rats [ J ]. Am JPhysi- ol Heart Circ Physiol, 2004, 287:H2 316.
  • 5Tang G, Wu L, Liang W, et al. Direct stimulation of KATp chan- nelsby exogenous and endogenous hydrogen sulfide in vascular smoothmuscle cells [J]. Mol Pharmacol, 2005, 68:1 757.
  • 6Zhang Z, Huang H, Liu P, et al. Hydrogen sulfide contributesto cardioprotection during ischemia-reperfusion injury by openingKATP channels [J]. Can J Physiol Pharmacol, 2007, 85:1 248.
  • 7Zhao W, Ndisang JF, Wang R. Modulation of endogenous produc- tionof HaS in rat tissues [J]. Can J Physiol Pharmacol, 2003, 81 : 848.
  • 8Webb GD, Lira LH, Oh VM, et al. Contractile and vasorelaxant ef- fectsof hydrogen sulfide and its biosynthesis in the human internal mammaryartery [J]. J Pharmacol Exp Ther, 2008, 324:876.
  • 9Johansen D, Ytrehus K, Baxter GF. Exogenous hydrogen sulfide (H2S) protects against regional myocardial ischemia-reperfusion in- jury Evidence for a role of KATP channels [ J ]. Basic Res Cardi- ol, 2006, 101:53.
  • 10Sivarajah A, Collino M, Yasin M, et al. Anti-apoptotic and anti-in- flammatory effects of hydrogen sulfide in a rat model of regional myo- cardial I/R [J]. Shock,2009, 31:267.

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中国心脏起搏与心电生理杂志
2011年 第3期

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