摘要
白细胞介素-6的可溶性受体(sIL-6R)在石英晶体微天平基片表面固定的量为297.08ng.cm-2。白细胞介素-6(IL-6)可以特异性结合sIL-6R,结合的量为33.70 ng.cm-2。IL-6的响应具有计量依赖性。根据上述分析,得到kass值为1.27×104(mol/L)-1s-1,kdiss值为1.94×10-4s-1,KD值为1.53×10-8mol/L。表明赤芍801能够增加IL-6与其受体的结合,从而影响IL-6及其受体的生物学活性。可以推断出IL-6与其受体的结合是赤芍801在体内的另一个作用靶点。
The immobilized amounts of interleukin-6 soluble receptor(sIL-6R) on the surface of quartz crystal microbalance(QCM) substrate are 297.08 ng·cm-2.interleukin-6(IL-6) can specifically bind to sIL-6R and the bound amounts are 33.70 ng·cm-2.The response for IL-6 is dose-dependent.Based on the analysis,the value for kass is 1.27×104(mol/L)-1s-1,the value for kdiss is 1.94×10-4s-1,the value for KD is 1.53×10-8 mol/L.Paeoniae radix 801 can enhance the binding amounts of IL-6/sIL-6R,which is dose-dependent.IL-6/sIL-6R is another action target of Paeoniae Radix 801 in vivo.
出处
《传感器与微系统》
CSCD
北大核心
2011年第6期45-48,共4页
Transducer and Microsystem Technologies
基金
国家自然科学基金资助项目(30972056
30911140278)
国家"863"计划资助项目(2007AA10Z430)
山东省优秀中青年科学家科研奖励基金资助项目(BS2009NY001)
山东省科技发展计划资助项目(2010GNC10960)