大豆GmPR10基因克隆与植物表达载体的构建

Cloning and plant expression vector construction of GmPR10 in soybean

为探明大豆中PR10蛋白质基因的抗大豆花叶病毒(SMV)作用机理,从抗SMV材料中克隆到GmPR10基因完整的cDNA序列,GmPR10基因的开放阅读框(ORF)全长477 bp,编码158个氨基酸。序列比对与进化树分析结果表明:GmPR10是大豆中一个新的PR10蛋白质基因,GmPR10基因在大豆的根、茎、叶中均能表达,接种大豆SMV后该基因在大豆叶片中被强烈诱导并高效表达,推测其可能使植物本身获得系统抗性以抵抗外来病原菌的侵袭。该研究构建了GmPR10基因的植物表达载体,为探究GmPR10基因在大豆抗病中的分子作用机理打下了基础。

In order to study the PR10 gene action mechanism for resistance to SMV（soybean mosaic virus）in soybean,the complete cDNA sequence of GmPR10 in resistant variety Kefeng No 1 was cloned.GmPR10 gene had a 477 bp ORF（open reading frame） and coded 158 amino acids.The sequence alignment and evolutionary tree clustering analysis results showed that GmPR10 was a new gene of PR10 protein,and expressed in root,stem and leaf of soybean.GmPR10 could be strongly induced and highly expressed in soybean leaves after SMV inoculation.Subsequently,a plant expression vector of GmPR10 was constructed,which could give grounds for exploring the molecular mechanism for disease resistance.