留兰香提取物体外抗猪细小病毒的作用

Antiviral activity of Mentha spicata Linn.extracts against porcine parvovirus in vitro

通过观察病毒引起的细胞病变效应(Cytopathogenic effect,CPE)和四甲基偶氮唑盐微量酶反应比色法(MTT)检测留兰香提取物抗猪细小病毒(PPV)活性,计算药物对病变的抑制率和半数抑制浓度(50%inhibitingconcentration,IC50),并从药物预防给药(抗病毒吸附)、直接杀灭及治疗给药(抑制病毒在细胞内的生物合成)3个方面分析留兰香提取物抗PPV活性的作用机制。结果显示:留兰香挥发油在这3种作用方式中对PPV均有抑制效果,其半数抑制浓度(IC50)分别为0.008 0 mg/ml、0.001 9 mg/ml、0.003 6 mg/ml,治疗指数(TI)分别为25.88、108.95、57.50;留兰香水提液和粗提物对PPV直接杀灭的半数有效浓度(IC50)分别为0.034 0 mg/ml、0.356 0mg/ml,治疗指数(TI)分别为79.18、8.37;留兰香水提液和粗提物抑制病毒在细胞内生物合成的半数有效浓度(IC50)分别为0.043 0 mg/ml、0.063 0 mg/ml,治疗指数(TI)分别为62.60、47.27,留兰香水提液和粗提物均无抗病毒吸附作用。表明留兰香挥发油在对PPV的3种作用方式中均有安全高效活性,留兰香水提液和粗提物对PPV侵入细胞无阻止作用,但在直接灭活和抑制其在细胞内的增殖方面均有较高的活性。

The experiment aimed to investigate antiviral activity of Mentha spicata Linn.extracts against porcine parvovirus（PPV） in vitro.The activity was measured by MTT assay and CPE（cytopathogenic effect）,based on which,inhibition ratio and median inhibiting concentration（IC50） were calculated.The antiviral mechanism was analyzed through three ways of drug administration,adding the extracts into cells before,after and simultaneous with PPV virus.The results demonstrated that the volatile oils of Mentha spicata Linn.had antivirus activities in the three reactions.Their median inhibiting concentrations（IC50） were 0.008 0 mg/ml,0.001 9 mg/ml and 0.003 6 mg/ml respectively.And the treatment indexes（TI） were 25.88,108.95 and 57.50 respectively.The aqueous extracts and rough extracts exhibited antivirus activities in the two ways（after and simultaneous with PPV）.When the two extracts were added into cells simultaneous with PPV,then their IC50 were 0.034 0 mg/ml and 0.356 0 mg/ml respectively,and TI were 79.18 and 8.37 respectively.When the two extracts were added into cells after PPV,IC50 were 0.043 0 mg/ml and 0.063 0 mg/ml respectively,and TI were 62.60 and 47.27 respectively.While they both had no antivirus activity when added into cells before PPV.It indicated that the volatile oils of Mentha spicata Linn.had safe and high-efficient antivirus activity in the three reactions.The aqueous and rough extracts could not keep out PPV virus into cells,but could efficiently kill them and control their multiplication in cells.