磷酸肌酸对阿霉素引起心肌损伤大鼠体内SOD、CAT和LPO水平的影响

Study on protective effect of phosphocreatine on myocardium injury induced by adriamycin in rats

目的观察阿霉素导致心脏毒性的大鼠体内超氧化物岐化酶(SOD),过氧化氢酶(CAT)和脂质过氧化物(LPO)水平的变化,及磷酸肌酸(CT)干预后对这些指标的影响及其机制。方法将30只SD大鼠随机分为3组。生理盐水对照组:每日腹腔注射与阿霉素等量的生理盐水;阿霉素组:实验中先每日腹腔注射生理盐水,第4日开始在腹腔注射生理盐水后30min给予阿霉素(3 mg/kg),隔日1次,共给药7次;阿霉素+磷酸肌酸钠组:实验中先每日腹腔注射磷酸肌酸钠(300 mg/kg),第4日开始在腹腔注射磷酸肌酸钠30 min后腹腔注射阿霉素(3 mg/kg),隔日1次,共给药7次。处死大鼠后心肌组织苏木精-伊红(HE)染色,光镜下观察各组大鼠心肌组织病理形态学变化,分别测定大鼠血清、心肌组织中LPO含量,SOD、CAT活性变化。结果磷酸肌酸干预阿霉素处理的大鼠后,降低了血清、心肌的LPO含量,增加了SOD、CAT活性,与阿霉素组相比差异有显著性统计学意义(P<0.05)。光镜下见阿霉素组心肌纤维排列系乱,加入磷酸肌酸钠和阿霉素组心肌细胞结构正常。结论磷酸肌酸能够对阿霉素引起的心肌损伤有保护作用,其主要机制是磷酸肌酸能抑制氧自由基引起的心肌脂质过氧化反应,减少LPO,增强抗氧化酶系SOD、CAT的活性。

Objective To study the protective effect and mechanism of phosphocreatine（CP） injection on the myocardiam injury induced by adriamycin（ADR） in rats.Methods Thirty SD rats were randomly divided into three groups.The control group,normal saline was ip injected at a dose of ADR everyday.The ADR group was given ip injection of normal saline once a day for 3 days,and ADR at a dose of 3 mg/kg 30 minutes after normal saline from the 4th day,once every other day for 7 times.ADR with CP treatment group was given ip injection of phosphocreatine Na at a dose of 300 mg/ kg 30 minutes after normal saline from the 4th day,once every other day for 7 times.The activities of superoxide dismutase（SOD）,catalase（CAT） and the contents of lipid peroxide（LPO） in serum and myocardial were determined.At the same time,the rat myocardial pathomorphism was determined. Results CP significantly reduced the contents of LPO,and increased the activities of SOD and CAT in myocardium and serum,when ADR treated rats were intervened by CP.It is significant to compare with the ADR group（P0.05）.The protection of myocardial pathological damage in therapy group was improved.Conclusion CP can inhibit myocardial lipid peroxidation induced by ADM.The mechanism maybe that CP can inhibited the activity of oxygen free radicals induced by ADM,thereby reduce the lever of LPO and protect the activities of SOD and CAT.