摘要
目的:探讨缺氧诱导因子-1α(HIF-1α)在原发性肝癌(PHC)血清中的表达及其与肝癌浸润转移的关系。方法:采用双抗体夹心ELISA法检测60例PHC患者和60例健康查体者血清中HIF-1α水平。结果:PHC组的血清HIF-1α水平为(161.14±80.79)ng/L,对照组为(22.67±8.47)ng/L,相比较有显著性差异(P<0.05);肿瘤远处转移组血清HIF-1α水平为(225.15±108.16)ng/L,无肿瘤远处转移组血清HIF-1α水平为(101.26±55.18)ng/L,两组相比较有显著性差异(P<0.05);有门脉癌栓组血清HIF-1α水平为(255.45±122.23)ng/L,无门脉癌栓组血清HIF-1α水平为(139.97±71.49)ng/L,两组相比较有显著性差异(P<0.05);肿瘤包膜组血清HIF-1α水平为(99.97±55.75)ng/L,无肿瘤包膜组血清HIF-1α水平为(187.36±91.52)ng/L,两组相比较有显著性差异(P<0.05);HIF-1α在不同年龄、性别、TNM分期、肿瘤直径大小患者血清中的表达差异无统计学意义。结论:HIF-1α在PHC患者血清中呈高表达,HIF-1α的高表达与有无肿瘤转移、有无癌栓和包膜有关。血清HIF-1α水平可以为预测PHC的浸润和转移的指标。
AIM： To study the expression of HIF-1α in serum of primary hepatocellular carcinoma（PHC） and effects on tumor invasion and metastasis.METHODS： The serum HIF-1α level from 60 cases of PHC patients and 60 cases of normal healthy individuals were detected by ELISA.RESULTS： The serum HIF-1α level of the PHC group was（161.14±80.79）pg/mL,the control group was（22.67±8.47）pg/mL,compared the different was significant（P0.05）;The serum HIF-1α level of distant metastasis of tumor group was（225.15± 108.16） pg/mL,the no distant metastasis of tumor group was（101.26 ± 55.18）pg/mL,compared the different was significant（P0.05）;The serum HIF-1α level of portal vein tumor thrombus group was（255.45±122.23）pg/mL,The no portal vein tumor thrombus group was（139.97±71.49）pg/mL,compared the different was significant（P0.05）;The serum HIF-1α level of tumor capsule group was（99.97±55.75）pg/mL,The no tumor capsule group was（187.36±91.52）pg/mL,compared the different was significant（P0.05）;The serum HIF-1α level was no significantly different among the different age,gender,TNM stage and tumor diameter.CONCLUSION： The serum HIF-1α has high expression in PHC.The serum HIF-1α expression has close relation with the whether distant metastasis of tumor,portal vein tumor thrombus and tumor capsule.The serum HIF-1α level can predict the invasion and metastasis of PHC.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第5期551-552,554,共3页
Chinese Journal of Cellular and Molecular Immunology
